Mitochondrial genome of Arcynopteryx dichroa (McLachlan, 1872) (Plecoptera: Perlodidae) and phylogenetic analysis

Abstract

To better understand the diversity and phylogeny of Perlodidae, we sequenced and annotated the complete mitochondrial genome (mitogenome) of Arcynopteryx dichroa. This mitogenome was 16,215 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), 2 ribosomal RNA unit genes (rRNAs), and a control region like other plecopteran. The nucleotide composition of A. dichroa mitochondrial genome obviously biases toward A and T. The A + T content of the whole mitochondrial genome, the PCGs, tRNAs, rRNAs, and the control region were: 69.3%, 67.6%, 69.8%, 71.3%, and 78.7%. Phylogenetic relationship based on the concatenated sequences of 13 PCGs and two ribosomal RNAs showed that the family Perlodidae and Chloroperlidae are sister relationship, family Perlidae being the sister group to the clade (Perlodidae + Chloroperlidae). The monophyly of the family Perlodidae is well supported but the subfamily Perlodinae and Isoperlinae are not monophyletic groups.

Keywords: Arcynopteryx dichroa; Mitochondrial genome; Perlodinae; phylogenetics analysis.

Figure 1.

Phylogenetic tree based on the concatenated nucleotide sequences of 13 PCGs and two ribosomal RNAs by maximum-likelihood (ML) methods from the complete mitochondrial genomes of Arcynopteryx dichroa and 28 other stoneflies. Scientific name is followed by the accession number for each species.