Correction: Aberrant DNA Damage Response Pathways May Predict the Outcome of Platinum Chemotherapy in Ovarian Cancer

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0117654.].

Fig 1. Changes in key molecules of the DDR pathways in ovarian carcinoma cell lines.

A2780/C30 cells (representative of the two OC cell lines) were treated (A) with cisplatin (0–100μg/ml) for 3h, or (B) with 100μg/ml cisplatin, subsequently incubated in drug-free medium for various time-periods (0–24h), or (C) with relatively small doses (0–15μg/ml) of cisplatin for up to 3h, and analyzed at the end of the treatment using confocal microscopy. Positive cells: cells with more than 5 foci per cell. The error bars represent standard deviation. In (D) typical images showing the key molecules under study using microscope analysis of A2780/C30 cells treated with 100μg/ml of cisplatin for 3h; upper images, immunofluorescence antigen staining; bottom images, cell nuclei labeled with DAPI. Typical images showing the same key molecules in the control/untreated A2780/C30 cells are also presented. (E) A2780/C30 cells were treated with carboplatin (0–300μg/ml) for 24h or (F) with 100μg/ml carboplatin for various time-periods (0–24h) and analyzed using confocal microscopy. The error bars represent standard deviation. All assays were performed in triplicate.