Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec 1;248(2):609-13.
doi: 10.1042/bj2480609.

Measurement of the matrix free Ca2+ concentration in heart mitochondria by entrapped fura-2 and quin2

G L Lukács  1 , A Kapus
Affiliations

Measurement of the matrix free Ca2+ concentration in heart mitochondria by entrapped fura-2 and quin2

G L Lukács et al. Biochem J. .

Abstract

A method was developed to monitor continuously the matrix free Ca2+ concentration ([Ca2+]m) of heart mitochondria by use of the fluorescent Ca2+ indicators, fura-2 and quin2. The acetoxymethyl esters of fura-2 and quin2 were accumulated in and hydrolysed by isolated mitochondria. An increase of the mitochondrial Ca content from 0.3 nmol/mg of protein to 6 nmol/mg corresponded to a rise of [Ca2+]m from 30 to 1000 nM. The results indicate that physiological fluctuations of the mitochondrial Ca content elicit changes of [Ca2+]m in that range which regulates the matrix dehydrogenases.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1971 Jan 22;42(2):298-305 - PubMed
    1. J Bioenerg Biomembr. 1984 Apr;16(2):101-13 - PubMed
    1. Biochemistry. 1976 AUG 24;15(17):3834-42 - PubMed
    1. FEBS Lett. 1980 Sep 22;119(1):1-8 - PubMed
    1. Rev Physiol Biochem Pharmacol. 1985;102:1-72 - PubMed

Publication types