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. 2021 Jul 23;26(15):4458.
doi: 10.3390/molecules26154458.

Phenolic Extract from Aralia nudicaulis L. Rhizomes Inhibits Cellular Oxidative Stresses

Quentin Lion  1 , Andre Pichette  1   2 , Mouadh Mihoub  1 , Vakhtang Mshvildadze  1   2 , Jean Legault  1   2
Affiliations

Phenolic Extract from Aralia nudicaulis L. Rhizomes Inhibits Cellular Oxidative Stresses

Quentin Lion et al. Molecules. .

Abstract

UV-B and IR-A radiation are important inducers of biological changes in skin involving ROS generation. The overloading of antioxidant defense mechanisms by ROS production could lead to photoaging and photocarcinogenesis processes. Various traditional usages are reported for Aralia nudicaulis L. extracts, including treatment of dermatological disorders. Antioxidant and anti-inflammatory properties have already been reported for other Aralia species possibly due to the presence of phenolic compounds. However, the phenolic composition and the potential activity of A. nudicaulis rhizomes extract against oxidative stress and UV/IR damages have not been investigated. The main aims of this study were to prepare a fraction enriched in phenolic compounds (FEPC) from A. nudicaulis rhizomes, to identify its major phenolic compounds and to assess its potential for protective effects against oxidative stress induced by UV-B, IR-A or inflammation. A quantitative LC-MS study of FEPC shows that chlorogenic, caffeic and protocatechuic acids are the main phenolic compounds present, with concentrations of 15.6%, 15.3% and 4.8% of the total composition, respectively. With a validated analytical method, those compounds were quantified over different stages of the growing period. As for biological potential, first this extract demonstrates antioxidant and anti-inflammatory activities. Furthermore, ROS generation induced by IR-A and UV-B were strongly inhibited by A. nudicaulis extract, suggesting that Aralia nudicaulis L. rhizome extract could protect dermal cells against oxidative stress induced by UV-B and IR-A.

Keywords: Aralia nudicaulis L.; IR-A; ROS; UV-B; enriched extract; fibroblasts; phenolic acids; quantitative analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
HPLC profiles of FEPC at 270 nm (A) and 320 nm (C); and HPLC profile of protocatechuic acid (PA), chlorogenic acid (ChA) and caffeic acid (CA) standards mix at 270 nm (B) and 320 nm (D).
Figure 2
Figure 2
HPLC quantitative pattern of the three main phenolic acid (mean ± SD): chlorogenic acid (ChA), caffeic acid (CA) and protocatechuic acid (PA) during the growing season of A. nudicaulis L. Significative differences of each phenolic acids between the vegetation stages are indicated by (*) according to Duncan’s test (p < 0.05).
Figure 3
Figure 3
Evaluation of protective effect of FEPC and positive controls (A) following UV-B and IR-A irradiation (4700 mJ/cm2) on human skin fibroblast (WS-1). Inhibition of DHR-123 fluorescence (B) of irradiated-cells treated or not with FEPC, protocatechuic acid, chlorogenic acid and caffeic acid analysed by Image J software. This result is representative of three independent experiments.
See this image and copyright information in PMC

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