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. 2021 Jul 24;26(15):4463.
doi: 10.3390/molecules26154463.

One-Step Extraction of Olive Phenols from Aqueous Solution Using β-Cyclodextrin in the Solid State, a Simple Eco-Friendly Method Providing Photochemical Stability to the Extracts

Aurélia Malapert  1 , Emmanuelle Reboul  2 , Olivier Dangles  1 , Alain Thiéry  3 , N'nabinty Sylla  4 , Valérie Tomao  1
Affiliations

One-Step Extraction of Olive Phenols from Aqueous Solution Using β-Cyclodextrin in the Solid State, a Simple Eco-Friendly Method Providing Photochemical Stability to the Extracts

Aurélia Malapert et al. Molecules. .

Abstract

The extraction of phenolic compounds from olive mill wastes is important, not only to avoid environmental damages, but also because of the intrinsic value of those biophenols, well-known for their high antioxidant potential and health benefits. This study focuses on tyrosol (Tyr) and hydroxytyrosol (HT), two of the main phenolic compounds found in olive mill wastes. A new, simple, and eco-friendly extraction process for the removal of phenolic compounds from aqueous solutions using native β-cyclodextrin (β-CD) in the solid state has been developed. Several β-CD/biophenol molar ratios and biophenol concentrations were investigated, in order to maintain β-CD mostly in the solid state while optimizing the extraction yield and the loading capacity of the sorbent. The extraction efficiencies of Tyr and HT were up to 61%, with a total solid recovery higher than 90% using an initial concentration of 100 mM biophenol and 10 molar equivalents of β-CD. The photochemical stability of the complexes thus obtained was estimated from ∆E*ab curve vs. illumination time. The results obtained showed that the phenols encapsulated into solid β-CD are protected against photodegradation. The powder obtained could be directly developed as a safe-grade food supplement. This simple eco-friendly process could be used for extracting valuable biophenols from olive mill wastewater.

Keywords: extraction; hydroxytyrosol; tyrosol; β-cyclodextrin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Time-dependence of tyrosol (A) and hydroxytyrosol (B) concentration according to the β-CD/phenol molar ratio.
Figure 2
Figure 2
Time-dependence of the biophenol concentration in a concentrated aqueous solution according to the β-CD/phenol molar ratio of 10:1.
Figure 3
Figure 3
∆E*ab values of β-CD, HT, Tyr, HT+β-CD, Tyr+β-CD during photochemical degradation.
Figure 4
Figure 4
Scanning electron micrographs of β-CD (A) and the powder recovered after extraction of Tyr from a 100 mM aqueous solution (10:1 β-CD/Tyr molar ratio) (B) (Magnification: ×140).
See this image and copyright information in PMC

References

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