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. 2022 Jan;29(2):2214-2226.
doi: 10.1007/s11356-021-15825-y. Epub 2021 Aug 7.

Melissa officinalis L. ameliorates oxidative stress and inflammation and upregulates Nrf2/HO-1 signaling in the hippocampus of pilocarpine-induced rats

Hagar N Abd Allah  1 , Manal Abdul-Hamid  1 , Ayman M Mahmoud  2   3 , Eman S Abdel-Reheim  1
Affiliations

Melissa officinalis L. ameliorates oxidative stress and inflammation and upregulates Nrf2/HO-1 signaling in the hippocampus of pilocarpine-induced rats

Hagar N Abd Allah et al. Environ Sci Pollut Res Int. 2022 Jan.

Abstract

Epilepsy is characterized by recurrent epileptic seizures, and its effective management continues to be a therapeutic challenge. Oxidative stress and local inflammatory response accompany the status epilepticus (SE). This study evaluated the effect of Melissa officinalis extract (MOE) on oxidative stress, inflammation, and neurotransmitters in the hippocampus of pilocarpine (PILO)-administered rats, pointing to the involvement of Nrf2/HO-1 signaling. Rats received PILO via intraperitoneal administration and were treated with MOE for 2 weeks. MOE prevented neuronal loss; decreased lipid peroxidation, Cox-2, PGE2, and BDNF; and downregulated glial fibrillary acidic protein in the hippocampus of PILO-treated rats. In addition, MOE enhanced GSH and antioxidant enzymes, upregulated Nrf2 and HO-1 mRNA abundance, and increased the nuclear translocation of Nrf2 in the hippocampus of epileptic rats. Na+/K+-ATPase activity and GABA were increased, and glutamate and acetylcholine were decreased in the hippocampus of epileptic rats treated with MOE. In conclusion, MOE attenuated neuronal loss, oxidative stress, and inflammation; activated Nrf2/HO-1 signaling; and modulated neurotransmitters, GFAP, and Na+/K+-ATPase in the hippocampus of epileptic rats. These findings suggest that M. officinalis can mitigate epileptogenesis, pending further studies to explore the exact underlying mechanisms.

Keywords: GABA; Inflammation; Lemon balm; Nrf2; Oxidative stress; Status epilepticus.

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References

    1. Al-Bishri WM, Abdel-Reheim ES, Zaki AR (2017) Purslane protects against the reproductive toxicity of carbamazepine treatment in pilocarpine-induced epilepsy model. Asian Pac J Trop Biomed 7:339–346 - DOI
    1. Alese OO, Mabandla MV (2019) Upregulation of hippocampal synaptophysin, GFAP and mGluR3 in a pilocarpine rat model of epilepsy with history of prolonged febrile seizure. J Chem Neuroanat 100:101659 - DOI
    1. Awad R, Muhammad A, Durst T, Trudeau VL, Arnason JT (2009) Bioassay-guided fractionation of lemon balm (Melissa officinalis L.) using an in vitro measure of GABA transaminase activity. Phytother Res : PTR 23:1075–1081 - DOI
    1. Baird L, Yamamoto MJM, Biology C (2020) The molecular mechanisms regulating the KEAP1-NRF2 pathway. Mol Cell Biol 40:e00099–20
    1. Bancroft JD, Gamble M (2008) Theory and practice of histological techniques. Elsevier Health Sciences, Philadelphia

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