Hybridoma cultivation in defined serum-free media: growth-supporting substances. IV. Lipids and serum albumin

Abstract

The effect of linoleic acid on growth of hybridomas in serum-free medium was studied. The optimum growth-supporting concentration of linoleic acid was 10 micrograms/ml. Omission of linoleic acid resulted in decreased growth. Hybridoma cells cultivated for 3 days without the fatty acid represented 52-65% of the number of cells growing with the fatty acid. Cholesterol (1 microgram/ml) and dipalmitoyl lecithin (3 micrograms/ml) also stimulated hybridoma growth when medium without linoleic acid was used. However, these substances did not enhance the effect of linoleic acid. Fatty acid-free serum albumin had no effect on hybridoma growth at the concentration (1 mg/ml) used for binding of lipids.