Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;32(2):15-28.
doi: 10.21315/tlsr2021.32.2.2. Epub 2021 Jun 29.

DNA Barcoding of Andaliman (Zanthoxylum acanthopodium DC) from North Sumatra Province of Indonesia Using Maturase K Gene

Cicik Suriani  1 , Eko Prasetya  1 , Tri Harsono  1 , Johannes Manurung  1 , Hary Prakasa  1 , Dina Handayani  1 , Miftahul Jannah  2 , Yuanita Rachmawati  3
Affiliations

DNA Barcoding of Andaliman (Zanthoxylum acanthopodium DC) from North Sumatra Province of Indonesia Using Maturase K Gene

Cicik Suriani et al. Trop Life Sci Res. 2021 Jun.

Abstract

Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum acanthopodium is a member of Rutaceae family widely found in northern Sumatra, Indonesia. The aim of this study was to barcode Z. acanthopodium in North Sumatra province, Indonesia based on cpDNA maturase K (matK). Samples were collected in seven localities across six regions of North Sumatra province. Phylogenetic analysis was conducted using Maximum Likelihood method. The results of phylogenetic analysis indicate that Z. acanthopodium is a monophyletic group that is derived from a common ancestor. The results of the phylogenetic tree construction show that there is a grouping of accession between Z. acanthopodium species separate from other species in the Zanthoxylum genus as well as those of the Rutaceae family. The results showed that cpDNA matK marker can effectively be used as DNA barcoding to identify Z. acanthopodium.

Keywords: DNA Barcoding; Maturase K; North Sumatra; Z. acanthopodium.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Sampling locations in North Sumatra, Indonesia at seven points in six regencies. (Source: Google Map and processed using ArcGis software).
Figure 2
Figure 2
Visualisation of PCR results of matK gene sequences with agarose: (3) South Tapanuli; (4) North Tapanuli; (5) Humbang Hasudutan; (11) Toba Samosir 1; (6) Dairi; (9) Simalungun; (10) Toba Samosir 2.
Figure 3
Figure 3
The phylogenetic tree of matK sequence from Z. acanthopodium and outgroup (species in the Zanthoxylum genus and Rutaceae family) as a result of reconstruction using the Maximum Likelihood method based on the kimura-2-parameter model. Branching is analysed with a bootstrap value of 1000x.
See this image and copyright information in PMC

References

    1. Arun K, Paridhavi M. An ethno botanical phytochemical and pharmacological utilization of widely distributed species Zanthoxylum: A comprehensive overview. International Journal of Pharmaceutical Invention. 2012;2(1):24–35.
    1. Bayer RJ, Mabberley DJ, Morton C, Miller CH, Sharma IK, Pfeil BE, Rich S, Hitchcock R, Sykes S. A molecular phylogeny of the orange subfamily (Rutaceae: Aurantioideae) using nine cpDNA sequences. American Journal of Botany. 2009;96(3):668–685. doi: 10.3732/ajb.0800341. - DOI - PubMed
    1. Borsch T, Hilu KW, Quandt D, Wilde V, Neinhuis C, Barthlott W. Noncoding plastid trnT-trnF sequences reveal a well resolved phylogeny of basal angiosperms. Journal of Evolutionary Biology. 2003;16(4):558–576. doi: 10.1046/j.1420-9101.2003.00577.x. - DOI - PubMed
    1. Chyau CC, Mau JL, Wu CM. Characteristics of the steam-distilled oil and carbon dioxide extract of Zanthoxylum simulans fruits. Journal of Agricultural and Food Chemistry. 1996;44(4):1096–1099. doi: 10.1021/jf950577d. - DOI
    1. Clegg MT, Durbin ML. Molecular approaches to the study of plant biosystematics. Australian Systematic Botany. 1990;3(1):1–8. doi: 10.1071/SB9900001. - DOI

LinkOut - more resources