Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Aug;1(8):e183.
doi: 10.1002/cpz1.183.

Models of Inflammation: Carrageenan Air Pouch

Jill C Fehrenbacher  1 , Kenneth E McCarson  2
Affiliations

Models of Inflammation: Carrageenan Air Pouch

Jill C Fehrenbacher et al. Curr Protoc. 2021 Aug.

Abstract

The subcutaneous air pouch is an in vivo model that can be used to study the components of acute and chronic inflammation, the resolution of the inflammatory response, the oxidative stress response, and potential therapeutic targets for treating inflammation. Injection of irritants into an air pouch in rats or mice induces an inflammatory response that can be quantified by the volume of exudate produced, the infiltration of cells, and the release of inflammatory mediators. The model presented in this article has been extensively used to identify potential anti-inflammatory drugs. © 2021 Wiley Periodicals LLC. Basic Protocol: Air pouch model in the rat Alternate Protocol: Air pouch model in the mouse.

Keywords: PGE2; TNF alpha; air pouch; animal model; carrageenan; edema; inflammation; inflammatory mediator; oxidative stress.

PubMed Disclaimer

References

Literature Cited

    1. Ajayi, A. M., Badaki, V. B., Ariyo, O. O., Ben-Azu, B., Asejeje, F. O., & Adedapo, A. D. A. (2020). Chrysophyllum albidum fruit peel attenuates nociceptive pain and inflammatory response in rodents by inhibition of pro-inflammatory cytokines and COX-2 expression through suppression of NF-kappaB activation. Nutrition Research, 77, 73-84. doi: 10.1016/j.nutres.2020.03.004.
    1. Ariel, A., & Serhan, C. N. (2007). Resolvins and protectins in the termination program of acute inflammation. Trends in Immunology, 28, 176-183. doi: 10.1016/j.it.2007.02.007.
    1. Baliou, S., Sofopoulos, M., Goulielmaki, M., Spandidos, D. A., Ioannou, P., Kyriakopoulos, A. M., & Zoumpourlis, V. (2021). Bromamine T, a stable active bromine compound, prevents the LPS-induced inflammatory response. International Journal of Molecular Medicine, 47(4). doi: 10.3892/ijmm.2021.4870.
    1. Castro, A. G., Esaguy, N., Macedo, P. M., Aguas, A. P., & Silva, M. T. (1991). Live but not heat-killed mycobacteria cause rapid chemotaxis of large numbers of eosinophils in vivo and are ingested by the attracted granulocytes. Infection and Immunity, 59, 3009-3014. doi: 10.1128/iai.59.9.3009-3014.1991.
    1. Chen, C. L., Cheng, M. H., Kuo, C. F., Cheng, Y. L., Li, M. H., Chang, C. P., … Lin, Y. S. (2018). Dextromethorphan attenuates NADPH oxidase-regulated glycogen synthase kinase 3beta and NF-kappaB activation and reduces nitric oxide production in group a streptococcal infection. Antimicrobial Agents and Chemotherapy, 62(6), e02045. doi: 10.1128/AAC.02045-17.

LinkOut - more resources