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. 2021 Aug 9;16(8):e0255829.
doi: 10.1371/journal.pone.0255829. eCollection 2021.

The effects of post-operative oxygen supply on blood oxygenation and acid-base status in rats anaesthetized with fentanyl/fluanisone and midazolam

Leander Gaarde  1 , Stefanie Kolstrup  2 , Peter Bollen  2
Affiliations

The effects of post-operative oxygen supply on blood oxygenation and acid-base status in rats anaesthetized with fentanyl/fluanisone and midazolam

Leander Gaarde et al. PLoS One. .

Abstract

In anaesthetic practice the risk of hypoxia and arterial blood gas disturbances is evident, as most anaesthetic regimens depress the respiratory function. Hypoxia may be extended during recovery, and for this reason we wished to investigate if oxygen supply during a one hour post-operative period reduced the development of hypoxia and respiratory acidosis in rats anaesthetized with fentanyl/fluanisone and midazolam. Twelve Sprague Dawley rats underwent surgery and were divided in two groups, breathing either 100% oxygen or atmospheric air during a post-operative period. The peripheral blood oxygen saturation and arterial acid-base status were analyzed for differences between the two groups. We found that oxygen supply after surgery prevented hypoxia but did not result in a significant difference in the blood acid-base status. All rats developed respiratory acidosis, which could not be reversed by supplemental oxygen supply. We concluded that oxygen supply improved oxygen saturation and avoided hypoxia but did not have an influence on the acid-base status.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1
Vital signs with oxygen saturation (a), heart rate (b), respiratory rate (c) and rectal temperature (d) of rats after surgery under fentanyl/fluanisone and midazolam anaesthesia. T0 represents measurements immediately after surgery. Measurements 30 and 60 minutes after surgery are indicated by T30 or T60 respectively. Oxygen supply is indicated by +O2 (with oxygen supply) and -O2 (without oxygen supply). Symbol shape indicates +O2 group for circle and -O2 group for square. In (a) **** indicates p<0.0001 for T30(-O2) and T60(-O2) versus all other measure points. In (c) * indicates p<0.05 for T0 versus T60(-O2) and T30(+O2) versus T60(-O2), and in (d) *** indicates p<0.001 for T0 versus T60(-O2). HR: Heart rate, RR: Respiratory rate, Temp: Temperature. Values are means ± standard deviations.
Fig 2
Fig 2
Blood acid-base status with pH (a), pCO2 (b), HCO3- (c) and pO2 (d) of rats after surgery under fentanyl/fluanisone and midazolam anaesthesia. T0 represents measurements immediately after surgery. Measurements 60 minutes (T60) after surgery are indicated by +O2 (with oxygen supply) and -O2 (without oxygen supply). Symbol shape indicates +O2 group for circle and -O2 group for square. In (a) ** indicates p<0.01 for T0 versus T60(+O2 and -O2). In (b) * indicates p<0.05 for T0 versus T60(+O2) and ** p<0.01 for T0 versus T60(-O2). In (c) ** indicates p<0.01 for T0 versus T60(-O2) and in (d) **** indicates p<0.0001 for T60(-O2) versus T0 and T60(+O2). Values are means ± standard deviations.
See this image and copyright information in PMC

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