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. 2021 Jul 19;13(7):1401.
doi: 10.3390/v13071401.

Laboratory Findings in Patients with Probable Dengue Diagnosis from an Endemic Area in Colombia in 2018

Jenny C Cardenas  1   2 , Sandra Y Giraldo-Parra  2 , Maria U Gonzalez  1   3 , Lady Y Gutierrez-Silva  1   4 , Lucy Jaimes-Villamizar  1   5 , Alba L Roa-Parra  1   6 , Daisy J Carvajal  1   6 , Hugo O Valdivia  7 , Juan F Sanchez  7 , Tonya M Colpitts  8 , Berlin Londono-Renteria  1
Affiliations

Laboratory Findings in Patients with Probable Dengue Diagnosis from an Endemic Area in Colombia in 2018

Jenny C Cardenas et al. Viruses. .

Abstract

As demonstrated with the novel coronavirus pandemic, rapid and accurate diagnosis is key to determine the clinical characteristic of a disease and to improve vaccine development. Once the infected person is identified, hematological findings may be used to predict disease outcome and offer the correct treatment. Rapid and accurate diagnosis and clinical parameters are pivotal to track infections during clinical trials and set protection status. This is also applicable for re-emerging diseases like dengue fever, which causes outbreaks in Asia and Latin America every 4 to 5 years. Some areas in the US are also endemic for the transmission of dengue virus (DENV), the causal agent of dengue fever. However, significant number of DENV infections in rural areas are diagnosed solely by clinical and hematological findings because of the lack of availability of ELISA or PCR-based tests or the infrastructure to implement them in the near future. Rapid diagnostic tests (RDT) are a less sensitive, yet they represent a timely way of detecting DENV infections. The purpose of this study was to determine whether there is an association between hematological findings and the probability for an NS1-based DENV RDT to detect the DENV NS1 antigen. We also aimed to describe the hematological parameters that are associated with the diagnosis through each test.

Keywords: PCR; dengue virus; hemogram; rapid diagnosis.

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Conflict of interest statement

The authors declare no conflict of interest. Disclaimer: The views expressed in this article reflect the results of research conducted by the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. Copyright: Several authors of this paper are employees of the U.S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government.’ Title 17 U.S.C. §101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person’s official duties.

Figures

Figure 1
Figure 1
Map of the study site located the State of Norte de Santander, Colombia.
Figure 2
Figure 2
Leukocyte differential (A) Neutrophiles, (B) Eosinophils, (C) Monocytes, (D) Basophiles and (E) Lymphocytes reported in percentage of cells compared between patients with positive or negative DENV-NS1-Ag tests.
See this image and copyright information in PMC

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