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. 2022 Feb;110(2):331-340.
doi: 10.1002/jbm.a.37291. Epub 2021 Aug 10.

A collagen-silica-based biocomposite for potential application in bone tissue engineering

María Alvarez Echazú  1   2   3 , Sandra Renou  2 , Gisela Alvarez  1   4 , Martin Desimone  1   4 , Daniel Olmedo  2   3
Affiliations

A collagen-silica-based biocomposite for potential application in bone tissue engineering

María Alvarez Echazú et al. J Biomed Mater Res A. 2022 Feb.

Abstract

Bone is a hierarchical material that has inspired the design of biopolymer-derived biocomposites for tissue engineering purposes. The present study sought to synthesize and perform the physicochemical characterization and biocompatibility of a collagen-silica-based biocomposite for potential application in bone tissue engineering. Ultrastructure, biodegradability, swelling behavior, and biocompatibility properties were analyzed to gain insight into the advantages and limitations to the use of this biomaterial as a bone substitute. Scanning electron microscopy analysis showed a packed-collagen fibril matrix and silica particles in the biocomposite three-dimensional structure. As shown by analysis of in vitro swelling behavior and biodegradability, it would seem that the material swelled soon after implantation and then suffered degradation. Biocompatibility properties were analyzed in vivo 14-days postimplantation using an experimental model in Wistar rats. The biocomposite was placed inside the hematopoietic bone marrow compartment of both tibiae (n = 16). Newly formed woven bone was observed in response to both materials. Unlike the pure-collagen-tissue interface, extensive areas of osseointegration were observed at the biocomposite-tissue interface, which would indicate that silica particles stimulated new bone formation. Agglomerates of finely particulate material with no inflammatory infiltrate or multinucleated giant cells were observed in the bone marrow implanted with the biocomposite. The biocomposite showed good biocompatibility properties. Further studies are necessary to evaluate their biological behavior over time.

Keywords: biocompatibility; biocomposite; bone tissue engineering; collagen; silica.

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References

REFERENCES

    1. Li M, Zhang X, Jia W, et al. Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide. Mater Sci Eng C. 2019;104:110008.
    1. Soufdoost RS, Yazdanian M, Tahmasebi E, et al. In vitro and in vivo evaluation of novel Tadalafil/β-TCP/collagen scaffold for bone regeneration: a rabbit critical-size calvarial defect study. Biocybern Biomed Eng. 2019;39:789-796.
    1. Reznikov N, Shahar R, Weiner S. Bone hierarchical structure in three dimensions. Acta Biomater. 2014;10:3815-3826.
    1. Martínez C, Fernández C, Prado M, Ozols A, Olmedo DG. Synthesis and characterization of a novel scaffold for bone tissue engineering based on Wharton's jelly. Journal of Biomedical Materials Research Part A, 2017;105(4):1034-1045. https://doi.org/10.1002/jbm.a.35976
    1. Xia Z, Yu X, Jiang X, Brody H, Rowe D, Wei M. Fabrication and characterization of biomimetic collagen-apatite scaffolds with tunable structures for bone tissue engineering. Acta Biomaterialia, 2013;9(7):7308-7319. https://doi.org/10.1016/j.actbio.2013.03.038

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