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Multicenter Study
. 2021 Aug 10;6(62):eabf3733.
doi: 10.1126/sciimmunol.abf3733.

Immunophenotyping assessment in a COVID-19 cohort (IMPACC): A prospective longitudinal study

IMPACC Manuscript Writing Team; IMPACC Network Steering Committee
Collaborators
Multicenter Study

Immunophenotyping assessment in a COVID-19 cohort (IMPACC): A prospective longitudinal study

IMPACC Manuscript Writing Team et al. Sci Immunol. .

Abstract

The IMmunoPhenotyping Assessment in a COVID-19 Cohort (IMPACC) is a prospective longitudinal study designed to enroll 1000 hospitalized patients with COVID-19 (NCT04378777). IMPACC collects detailed clinical, laboratory and radiographic data along with longitudinal biologic sampling of blood and respiratory secretions for in depth testing. Clinical and lab data are integrated to identify immunologic, virologic, proteomic, metabolomic and genomic features of COVID-19-related susceptibility, severity and disease progression. The goals of IMPACC are to better understand the contributions of pathogen dynamics and host immune responses to the severity and course of COVID-19 and to generate hypotheses for identification of biomarkers and effective therapeutics, including optimal timing of such interventions. In this report we summarize the IMPACC study design and protocols including clinical criteria and recruitment, multi-site standardized sample collection and processing, virologic and immunologic assays, harmonization of assay protocols, high-level analyses and the data sharing plans.

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Figures

Fig. 1.
Fig. 1.. IMPACC sites and Core Labs.
The 15 IMPACC clinical sites are located within 12 states across the United States. Core Labs are located at seven sites, six of which are co-located with clinical sites. Assays conducted by each Core Lab are indicated in Fig. 4.
Fig. 2.
Fig. 2.. IMPACC study overview.
This schematic represents the IMPACC study design in which clinical data, mid-turbinate nasal swabs, and blood samples are collected at each indicated visit during and after hospitalization. EAs are only collected from intubated patients.
Fig. 3.
Fig. 3.. Sample processing pipeline and Core Lab assays.
Nasal swabs are used to measure viral titers, for viral genome sequencing and metagenomics, and for bulk nasal transcriptomics. Serum samples are used to measure inflammatory markers (Olink), anti-SARS and human CoV antibodies, and for untargeted proteomics and untargeted/targeted metabolomics. Whole blood is used for genome-wide association study (GWAS), WES, and CyTOF. Plasma is used for untargeted proteomics and untargeted/targeted metabolomics. PBMCs are used for bulk transcriptomics analysis. EAs are processed for bulk transcriptomics and CyTOF analyses.
Fig. 4.
Fig. 4.. Data flow from Core Lab to data analysis.
IMPACC Core Labs conduct the designated assays, QC, and preliminary data analysis before sending the validated datasets to the IMPACC CDCC for QA. After QC/QA, these data are provided to the DAWG for independent and integrated analyses to identify correlates of clinical outcomes, COVID-19 disease severity and progression, and multiomic signatures.
See this image and copyright information in PMC

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