A tale of two metrics: the EPA Risk Quotient Approach versus the delay in Population Growth Index for determination of pesticide risk to aquatic species

Abstract

The potential risk that two closely related insecticides, spinetoram and spinosad, posed to three Cladoceran species, Ceriodaphnia dubia, Daphnia pulex, and D. magna was determined using two approaches, the USEPA Risk Quotient method and the Delay in Population Growth Index (DPGI). Results of the RQ method showed that spinetoram posed a risk to all three species, but spinosad posed a risk only to C. dubia. The DPGI analysis showed that exposure to spinetoram resulted in populations of all three species being delayed ≥ 3 generation times. Exposure to the LC₅₀ and the lower 95% CL resulted in delayed populations while exposure to the upper 95% CL concentration of spinetoram resulted in no recovery of any of the three species over the course of the modeling exercise (88 d). Exposure to the lower and upper 95% Cl and the LC₅₀ of spinosad resulted in C. dubia populations being delayed ≥ 3 generations. D. pulex populations were not negatively affected after exposure to spinosad. D. magna populations were delayed ≥ 3 generations, but only after exposure to the upper 95% Cl of spinosad. These results illustrate that although the EPA risk quotient method indicated that spinetoram posed a risk to all three species and that spinosad only posed a risk to C. dubia, the DPGI showed that D. magna would be negatively affected by spinosad and none of the three species would reach a predetermined number of individuals after exposure to the upper 95% CL of spinetoram. Because the DPGI uses the 95% Cl as well as the LC₅₀ in its calculation and produces a measure of population growth it provides more detailed information in terms of the potential risk of pesticides to populations than the RQ method.

Keywords: Daphnia; Delay in Population Growth Index; EPA; Ecological risk assessment; Pesticides; Risk Quotient.