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. 2021:2351:165-179.
doi: 10.1007/978-1-0716-1597-3_9.

Low Input Targeted Chromatin Capture (Low-T2C)

Ilias Boltsis  1 , Karol Nowosad  1   2   3 , Rutger W W Brouwer  1   4 , Przemko Tylzanowski  2   5 , Wilfred F J van IJcken  1   4 , Danny Huylebroeck  1   6 , Frank Grosveld  7 , Petros Kolovos  8
Affiliations

Low Input Targeted Chromatin Capture (Low-T2C)

Ilias Boltsis et al. Methods Mol Biol. 2021.

Abstract

Targeted chromatin capture (T2C) is a 3C-based method and is used to study the 3D chromatin organization, interactomes and structural changes associated with gene regulation, progression through the cell cycle, and cell survival and development. Low input targeted chromatin capture (low-T2C) is an optimized version of the T2C protocol for low numbers of cells. Here, we describe the protocol for low-T2C, including all experimental steps and bioinformatics tools in detail.

Keywords: C-technologies; Chromatin; Chromatin conformation capture; Genome architecture; T2C; Three-dimensional (3D) organization; Topologically associating domains (TADs).

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