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Comment
. 2021 Aug 16;40(16):e108671.
doi: 10.15252/embj.2021108671.

Response to Veitia et al

Eunkyoung Shin  1 , Hanyong Jin  2 , Dae-Shik Suh  3 , Yongyang Luo  1 , Wooseok Song  2 , Hye-Jeong Ha  1 , Tae Heon Kim  4 , Yoonsoo Hahn  2 , Seogang Hyun  2 , Kangseok Lee  2 , Jeehyeon Bae  1
Affiliations
Comment

Response to Veitia et al

Eunkyoung Shin et al. EMBO J. .

Abstract

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Figures

Figure 1
Figure 1. Allelic imbalance of heterozygous FOXL2 transcripts in KGN cells
(A) Electropherograms from the Sanger sequencing of the locus of c.402C>G of FOXL2 from cDNA and gDNA from KGN cells are presented. The presented electropherograms are representative of eight independent experiments. The red dotted boxes indicate the 402C>G position. (B) Actual pyrograms and PyroMark Q48 software‐generated allele proportions of WT and variant FOXL2 mRNAs in KGN and COV434 cells from pyrosequencing experiments are presented. The data are provided as the mean ± SEM from two independent experiments. (C) Primer extension analysis performed on total RNA extracted from KGN cells transfected with an empty vector (control) or mutant (402G) FOXL2‐encoding plasmid. The expected WT (C) or mutated (G) nucleotides at position 402 are indicated by blue or red text and arrowheads, respectively. Enlarged images of signals from the 402 regions of FOXL2 transcripts are presented below.
See this image and copyright information in PMC

Comment on

References

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