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. 2021 Aug 17;53(4):439.
doi: 10.1007/s11250-021-02870-5.

Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan

Kainat Abid  1 , Sundas Bukhari  1 , Muhammad Asif  1 , Ayesha Sattar  2 , Maryam Arshad  2 , Munir Aktas  3 , Sezayi Ozubek  3 , Rehan Sadiq Shaikh  4 , Furhan Iqbal  5
Affiliations

Molecular detection and prevalence of Theileria ovis and Anaplasma marginale in sheep blood samples collected from Layyah district in Punjab, Pakistan

Kainat Abid et al. Trop Anim Health Prod. .

Abstract

Theileria ovis and Anaplasma marginale are intracellular pathogens affecting a wide range of animals, causing huge economic losses worldwide. The present study reports the molecular evidence of Theileria ovis and Anaplasma marginale in sheep blood samples (N = 218) collected from Layyah district in Punjab (Pakistan), where economy heavily relies on livestock. A 520 base pair fragment specific for 18S ribosomal RNA gene of Theileria ovis was PCR amplified in 23/218 (10.6%) sheep blood samples, while for Anaplasma marginale, a 265 base pair fragment specific for msp1b gene was generated in 15/218 (6.9%) sheep blood samples. Two blood samples were found co-infected (0.9%) with both parasites. Amplified PCR products of both parasites were confirmed by DNA sequencing and submitted to GenBank. Prevalence of both Theileria ovis (p = 0.3) and Anaplasma marginale (p = 0.4) varied non-significantly among the investigated sheep breeds. Tick burden on dogs present with sheep herds was found associated with Theileria ovis infection in sheep (p = 0.05). It was observed that lambs (p = 0.009), sheep in small herds (p = 0.04), and tick burden on dogs present with sheep herds (p = 0.01) were associated with Anaplasma marginale infection in sheep during the present study. In conclusion, we are reporting a higher prevalence of Theileria ovis than Anaplasma marginale in blood samples of sheep collected from Layyah district. Tick-infested dogs were found to be risk factors for the transmission of both pathogens in sheep, and tick control strategies should be extended to dogs associated with sheep herds in this area.

Keywords: Anaplasma marginale; Layyah district; PCR; Phylogenetic analysis; Sheep; Theileria ovis.

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References

    1. Ali, A., Khan, M.A., Zahid, H., Yaseen, P.M., Qayash, K.M., Nawab, J., Rehman, Z.U., Ateeq, M., Khan, S., Ibrahim, M., 2019. Seasonal dynamics, record of ticks infesting humans, wild and domestic animals and molecular phylogeny of Rhipicephalus microplus in Khyber Pakhtunkhwa Pakistan. Frontiers in Physiology, 10, 973.
    1. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., Lipman, D.J., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25, 3389-402. - DOI
    1. Altay, K., Dumanli, N., Aktas, M., 2007. Molecular identification, genetic diversity and distribution of Theileria and Babesia species inf ll ruminants. Veterinary Parasitology, 147, 161-165. - DOI
    1. Asif, M., Parveen, A., Ashraf, S., Hussain, M., Aktas, M., Ozubek, S., Shaikh, R.S., Iqbal, F., 2019. First report regarding the simultaneous molecular detection of Anaplasma marginale and Theileria annulata in equine blood samples collected from Southern Punjab in Pakistan. Acta Parasitologica, 65, 259-263. - DOI
    1. Ashraf, S., Parveen, A., Awais, M.M., Gillani, Q.A.U., Aktas, M., Ozubek, S., Iqbal, F., 2020. A Report on Molecular Detection and Phylogenetic Evaluation of Anaplasma marginale in Ticks and Blood Samples Collected from Cattle in District Layyah in Punjab (Pakistan). Current Microbiology 78(1), 274-281. - DOI

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