Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2021 Oct:206:55-59.
doi: 10.1016/j.thromres.2021.08.007. Epub 2021 Aug 13.

Morphological and functional alterations in endothelial colony-forming cells from recovered COVID-19 patients

José Antonio Alvarado-Moreno  1 , Jorge Davila-Moreno  2 , Victor Dominguez-Reyes  3 , Rodrigo Arreola-Diaz  3 , Irma Isordia-Salas  3 , Antonieta Chavez-Gonzalez  4 , Abraham Majluf-Cruz  3
Affiliations
Comment

Morphological and functional alterations in endothelial colony-forming cells from recovered COVID-19 patients

José Antonio Alvarado-Moreno et al. Thromb Res. 2021 Oct.
No abstract available

Keywords: COVID-19; Endothelial Colony-Forming Cells; Endothelial cell dysfunction; Endothelial cells; Thrombosis.

PubMed Disclaimer

Conflict of interest statement

Authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1
Frequency and identification of ECFCs in PB from recovered COVID-19 patients, healthy donors (Controls) and VTD patients. (A) The figure shows the number of ECFCs colonies obtained per 100 mL of MNCs seeded in culture plates. (B) Time to detection (days) of ECFCs per 1 × 108 MNCs seeded in culture plates. Results are expressed as mean ± SD of 15, 10 and 5 independent samples, respectively. *: P < 0.001, Controls and recovered COVID-19 patients vs. VTD patients. **: P < 0.001, Controls vs. recovered COVID-19 patients and VTD patients. Representative phase-contrast photomicrographs from 3 experiments in triplicate (4× magnification) of ECFCs colonies detected in culture from: Controls before (C) and after expansion (D); recovered COVID-19 patients before (E) and after expansion (F) and VTD patients before (G) and after expansion (H). Arrows indicate colony boundary, the typical morphology of a normal immature colony and after reseeding, they present a robust proliferative potential that leads to cell confluence. VTD patients present the same characteristics but with slow proliferation; in recovered COVID-19 patients, the colonies show obvious morphological alterations without proliferative capacity. Scale bar represents 200 μm.
Fig. 2
Fig. 2
Controls but not COVID-19 immature and VTD mature ECFCs form tubular structures in vitro. Representative phase-contrast photomicrograph from 3 experiments in triplicate (4× magnification) of an immature ECFCs culture in normal medium for Controls (A), and recovered COVID-19 patients (B); arrows indicate colony boundary. To evaluate angiogenic ability, were incubated 48 h with plasma from recovered COVID-19 patients, tubular structures with characteristics similar to vascular lumen were detected in Controls (C), arrows show these structures but not in recovered COVID-19 patients (D). Mature ECFCs (passage 3) from Controls (E) or VTD patients (H) were incubated 48 h in presence of plasma from healthy donors in Controls (F) and VTD patients ECFCs (I) or plasma of recovered COVID-19 patients in Controls (G) and VTD patients ECFCs (J). Presence of control and COVID-19 plasma, shows in ECFCs of Controls, similar effects as those found in immature ECFCs, with similar tubular structures when cells were incubated in the control and COVID-19 plasma (F) and (G). Arrows show structures similar to vascular lumen. In mature ECFC derived from VTD, control and COVID-19 plasma show a low ability to form tubular structures (I) and (J). Arrows show structures with deficiencies to form vascular structures. Scale bar represents 200 μm.
See this image and copyright information in PMC

Comment on

References

    1. World Health Organization . August 3, 2021. COVID-19 Weekly Epidemiological Update. Edition 51. published.
    1. Klok F.A., Kruip M.J.H.A., van der Meer N.J.M., et al. Confirmation of the high cumulative incidence of thrombotic complications in critically ill ICU patients with COVID-19: an updated analysis. Thromb. Res. 2020;191:148–150. doi: 10.1016/j.thromres.2020.04.041. - DOI - PMC - PubMed
    1. Thachil J., Tang N., Gando S., et al. ISTH interim guidance on recognition and management of coagulopathy in COVID-19. J. Thromb. Haemost. 2020;18:1023–1026. doi: 10.1111/jth.14810. - DOI - PMC - PubMed
    1. Critser P.J., Yoder. Endothelial colony forming cells role in neoangiogenesis and repair. Curr. Opin. Organ. Transplant. 2010;15:68–72. doi: 10.1097/MOT.0b013e32833454b5. - DOI - PMC - PubMed
    1. Mancuso P., Gidaro A., Gregato G., et al. Circulating endothelial progenitors are increased in Covid-19 patients and correlate with SARS-CoV-2 RNA in severe cases. J. Thromb. Haemost. 2020;6 doi: 10.1111/jth.15044. - DOI - PMC - PubMed