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. 2021 Jul 1;21(4):15.
doi: 10.1093/jisesa/ieab061.

The Influence of Gut Microbiota on the Fecundity of Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

Hanwen Li  1   2 , Changwei Zhao  1   2 , Yang Yang  1   2 , Zhixiong Zhou  1   2 , Jingwei Qi  1   2 , Chuanren Li  1   2
Affiliations

The Influence of Gut Microbiota on the Fecundity of Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae)

Hanwen Li et al. J Insect Sci. .

Abstract

The gut microbiota of insects usually plays an important role in the development and reproduction of their hosts. The fecundity of Henosepilachna vigintioctopunctata (Fabricius) varies greatly when they develop on different host plants. Whether and how the gut microbiota regulates the fecundity of H. vigintioctopunctata was unknown. To address this question, we used 16S rRNA sequencing to analyze the gut microbiomes of H. vigintioctopunctata adults fed on two host plant species (Solanum nigrum and Solanum melongena) and one artificial diet. The development of the ovaries and testes was also examined. Our results revealed that the diversity and abundance of gut microorganisms varied significantly in insects reared on different diets. The gut microbiota of H. vigintioctopunctata raised on the two host plants was similar, with Proteobacteria being the dominant phylum in both groups, whereas Firmicutes was the dominant phylum in the group reared on the artificial diet. The predominant microbiota in the S. nigrum group were Acinetobacter soli and Acinetobacter ursingii (Acinetobacter, Moraxellaceae); Moraxella osloensis (Enhydrobacter, Moraxellaceae); and Empedobacter brevis (Empedobacter, Weeksellaceae). The microbiota in this group are associated with high lipid metabolism. In addition, the beetles' ovaries and testes were more highly developed in the S. nigrum group than in the other two groups. These findings provide valuable information for elucidating the complex roles the gut microbiota play in the fecundity of H. vigintioctopunctata, and may also contribute to developing future novel control strategies involving this economically important pest.

Keywords: Henosepilachna vigintioctopunctata; diet; gut microorganism; reproduction.

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Figures

Fig. 1.
Fig. 1.
Diversity and abundance of the gut microbial communities in H. vigintioctopunctata examined via Rarefaction analysis (A), PcoA (B), ACE analysis (C), the Chao1 index (D), and the Shannon index (E).
Fig. 2.
Fig. 2.
Weighted UniFrac UPGMA tree of the gut microorganisms in H. vigintioctopunctata.
Fig. 3.
Fig. 3.
Abundance of different gut microbial taxa observed in the three groups using linear discriminant analysis effect size (LEfSe analysis).
Fig. 4.
Fig. 4.
Correlation between the microbes identified in the LEfSe analyses with Tax4Fun functional predictions.
Fig. 5.
Fig. 5.
Ovary and testis development in H. vigintioctopunctata adults reared on different diets (white bars indicate a scale of 1 mm).
See this image and copyright information in PMC

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