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. 2021 Nov;14(11):2265-2267.
doi: 10.1016/j.jcmg.2021.06.017. Epub 2021 Aug 18.

Intravascular Molecular-Structural Assessment of Arterial Inflammation in Preclinical Atherosclerosis Progression

Eric A Osborn, Giovanni J Ughi, Johan W Verjans, Zhonglie Piao, Edouard Gerbaud, Mazen Albaghdadi, Haitham Khraishah, Mohamad B Kassab, Richard A P Takx, Jie Cui, Adam Mauskapf, Changyu Shen, Robert W Yeh, Michael T Klimas, Ahmed Tawakol, Guillermo J Tearney, Farouc A Jaffer

Intravascular Molecular-Structural Assessment of Arterial Inflammation in Preclinical Atherosclerosis Progression

Eric A Osborn et al. JACC Cardiovasc Imaging. 2021 Nov.
No abstract available

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Figures

FIGURE 1
FIGURE 1. Intravascular Molecular-Structural NIRF-OCT Arterial Plaque Inflammation Imaging in Vivo
(A) Representative multimodal images at 12 weeks after balloon injury. X-ray angiography demonstrates mild atherosclerosis in the balloon-injured zone (white line). Longitudinal optical coherence tomography (OCT) and 1-dimensional (1D) average near-infrared fluorescence (NIRF) inflammation image overlay (yellow/green is high; blue is low). In vivo 2-dimensional (2D) near-infrared fluorescence maps reveal heterogeneous near-infrared fluorescence inflammation across the catheter pull back, corroborated by ex vivo fluorescence reflectance imaging (FRI) near-infrared fluorescence. (Scale = 5 mm.) (B) Serial intravascular near-infrared fluorescence–optical coherence tomography imaging. The color bar indicates regions of high (yellow/green) and low (blue) near-infrared fluorescence inflammation. Quantitative 1-dimensional near-infrared fluorescence and near-infrared fluorescence ratio (integrated near-infrared fluorescence signal at 16 weeks divided by 8 weeks) and 1-dimensional plaque burden (PB) plot and percent atheroma volume (PAV) ratio (16 weeks/8 weeks). (C) Baseline near-infrared fluorescence inflammation is associated with change in plaque burden plaque progression. Representative axial slice at 8 weeks showing high near-infrared fluorescence inflammatory protease activity followed by high intravascular ultrasound (IVUS) plaque progression from 8 weeks-12 weeks (v = vessel, fiducial). External elastic membrane (yellow) minus lumen (white) contours define the atheroma cross-sectional area (CSA). (Scale = 1 mm.) (D) Baseline near-infrared fluorescence inflammation is associated with change in percent atheroma volume plaque progression. Univariable regression per animal comparing 8-week near-infrared fluorescence inflammation and intravascular ultrasound change in percent atheroma volume plaque progression between 8 weeks and 12 or 16 weeks. **P < 0.01.
FIGURE 1
FIGURE 1. Intravascular Molecular-Structural NIRF-OCT Arterial Plaque Inflammation Imaging in Vivo
(A) Representative multimodal images at 12 weeks after balloon injury. X-ray angiography demonstrates mild atherosclerosis in the balloon-injured zone (white line). Longitudinal optical coherence tomography (OCT) and 1-dimensional (1D) average near-infrared fluorescence (NIRF) inflammation image overlay (yellow/green is high; blue is low). In vivo 2-dimensional (2D) near-infrared fluorescence maps reveal heterogeneous near-infrared fluorescence inflammation across the catheter pull back, corroborated by ex vivo fluorescence reflectance imaging (FRI) near-infrared fluorescence. (Scale = 5 mm.) (B) Serial intravascular near-infrared fluorescence–optical coherence tomography imaging. The color bar indicates regions of high (yellow/green) and low (blue) near-infrared fluorescence inflammation. Quantitative 1-dimensional near-infrared fluorescence and near-infrared fluorescence ratio (integrated near-infrared fluorescence signal at 16 weeks divided by 8 weeks) and 1-dimensional plaque burden (PB) plot and percent atheroma volume (PAV) ratio (16 weeks/8 weeks). (C) Baseline near-infrared fluorescence inflammation is associated with change in plaque burden plaque progression. Representative axial slice at 8 weeks showing high near-infrared fluorescence inflammatory protease activity followed by high intravascular ultrasound (IVUS) plaque progression from 8 weeks-12 weeks (v = vessel, fiducial). External elastic membrane (yellow) minus lumen (white) contours define the atheroma cross-sectional area (CSA). (Scale = 1 mm.) (D) Baseline near-infrared fluorescence inflammation is associated with change in percent atheroma volume plaque progression. Univariable regression per animal comparing 8-week near-infrared fluorescence inflammation and intravascular ultrasound change in percent atheroma volume plaque progression between 8 weeks and 12 or 16 weeks. **P < 0.01.
See this image and copyright information in PMC

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