Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan;19(1):133-154.
doi: 10.1007/s12015-021-10221-y. Epub 2021 Aug 22.

Combining Bone Collagen Matrix with hUC-MSCs for Application to Alveolar Process Cleft in a Rabbit Model

Xue-Cheng Sun  1   2 , Hu Wang  1   3 , Dan Zhang  1   3 , Jian-Hui Li  1   3 , Li-Qiang Yin  4 , Yu-Fang Yan  4 , Xu Ma  5   6   7 , Hong-Fei Xia  8   9   10
Affiliations

Combining Bone Collagen Matrix with hUC-MSCs for Application to Alveolar Process Cleft in a Rabbit Model

Xue-Cheng Sun et al. Stem Cell Rev Rep. 2023 Jan.

Abstract

Background: Most materials used clinically for filling severe bone defects either cannot induce bone re-generation or exhibit low bone conversion, therefore, their therapeutic effects are limited. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit good osteoinduction. However, the mechanism by which combining a heterogeneous bone collagen matrix with hUC-MSCs to repair the bone defects of alveolar process clefts remains unclear.

Methods: A rabbit alveolar process cleft model was established by removing the bone tissue from the left maxillary bone. Forty-eight young Japanese white rabbits (JWRs) were divided into normal, control, material and MSCs groups. An equal volume of a bone collagen matrix alone or combined with hUC-MSCs was implanted in the defect. X-ray, micro-focus computerized tomography (micro-CT), blood analysis, histochemical staining and TUNEL were used to detect the newly formed bone in the defect area at 3 and 6 months after the surgery.

Results: The bone formation rate obtained from the skull tissue in MSCs group was significantly higher than that in control group at 3 months (P < 0.01) and 6 months (P < 0.05) after the surgery. The apoptosis rate in the MSCs group was significantly higher at 3 months after the surgery (P < 0.05) and lower at 6 months after the surgery (P < 0.01) than those in the normal group.

Conclusions: Combining bone collagen matrix with hUC-MSCs promoted the new bone regeneration in the rabbit alveolar process cleft model through promoting osteoblasts formations and chondrocyte growth, and inducing type I collagen formation and BMP-2 generation.

Keywords: Alveolar process cleft; Bone collagen matrix; Micro-CT; Young rabbit; hUC-MSCs.

PubMed Disclaimer

References

    1. Cohen, M., Figueroa, A. A., Haviv, Y., Schafer, M. E., & Aduss, H. (1991). Iliac versus cranial bone for secondary grafting of residual alveolar clefts. Plastic and Reconstructive Surgery, 87(3), 423–427.
    1. Tai, C. C., Sutherland, I. S., & McFadden, L. (2000). Prospective analysis of secondary alveolar bone grafting using computed tomography. Journal of Oral and Maxillofacial Surgery, 58(11), 1241–1249.
    1. LaRossa, D., Buchman, S., Rothkopf, D. M., Mayro, R., & Randall, P. (1995). A comparison of iliac and cranial bone in secondary grafting of alveolar clefts. Plastic and Reconstructive Surgery, 96(4), 789–797.
    1. Rosenthal, R. K., Folkman, J., & Glowacki, J. (1999). Demineralized bone implants for nonunion fracture, bone cysts, and fibous lesins. Clinical Orthopaedics and Related Research, 364, 61–69.
    1. Al-Asfour, A., Farzad, P., Andersson, L., Joseph, B., & Dahlin, C. (2014). Host tissue reactions of non-demineralized autogenic and xenogenic dentin blocks implanted in a non-osteogenic environment. An experimental study in rabbits. Dental Traumatology, 30(3), 198–203.

Publication types

LinkOut - more resources