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Comment
. 2021 Aug 4:11:702709.
doi: 10.3389/fcimb.2021.702709. eCollection 2021.

Commentary: Development of a Comparative European Orthohantavirus Microneutralization Assay With Multi-Species Validation and Evaluation in a Human Diagnostic Cohort

Jan Clement  1   2 , Jan Groen  3 , Guido van der Groen  4 , Marc Van Ranst  1   2 , Piet Maes  1   2 , Albertus D M E Osterhaus  3
Affiliations
Comment

Commentary: Development of a Comparative European Orthohantavirus Microneutralization Assay With Multi-Species Validation and Evaluation in a Human Diagnostic Cohort

Jan Clement et al. Front Cell Infect Microbiol. .
No abstract available

Keywords: Netherlands; Puumala orthohantavirus; Seoul orthohantavirus; acute kidney injury; biomolecular diagnosis; haemorrhagic fever with renal syndrome; rats; serodiagnosis.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
IFA: immuno-fluorescence assay or immuno-fluorescence test (IFT). ELISA, enzyme-linked immunosorbent assay. CTB ELISA, complex-trapping blocking, an inhibition ELISA variant. Closed circles: 1980s laboratory rat-acquired human hantavirus infections. Open circles: non-laboratory rat-acquired or “wild” human hantavirus infections, mostly being 1980s PUUV-induced, except for two “wild” cases (arrows), being 1980s wild rat-induced SEOV infections. Thick arrow: Dutch farmer, thin arrow: Belgian homeless vagabond, both with wild rat-exposure. In these two cases, SEOV infection was missed in IFA, showing surprisingly high cross-reacting PUUV titers (A), but confirmed, albeit with lower titers, in ELISA (B), and unmistakably ascertained in CTB-ELISA (C). Consequently, these two cases would have been mistaken for PUUV infections, if relying only on IFT/IFA. Adapted from Groen et al., 1991. Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company.
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Comment on

References

    1. Clement J. (2015). “Acute Kidney Injury and Hantavirus Disease,” in Oxford Textbook of Clinical Nephrology, 4th edition. Eds. Turner N., Lameire N., Goldsmith D., Winearls C., Himmelfarb J., Remuzzi G. (Oxford, UK: Oxford University Press; ), 2059–2066. 10.1093/med/9780199592548.001.0001 - DOI
    1. Clement J., Heyman P., McKenna P., Colson P., Avsic-Zupanc T. (1997). The Hantaviruses of Europe: From the Bedside to the Bench. Emerg. Infect. Dis. 3 (2), 205–211. 10.3201/eid0302.970218 - DOI - PMC - PubMed
    1. Clement J., LeDuc J. W., Lloyd G., Reynes J. M., McElhinney L., Van Ranst M., et al. (2019). Wild Rats, Laboratory Rats, Pet Rats: Global Seoul Hantavirus Disease Revisited. Viruses 11 (7), 1–29. 10.3390/v11070652 - DOI - PMC - PubMed
    1. Clement J., van der Groen G. (1987). Acute Hantavirus Nephropathy in Belgium: Preliminary Results of a Sero-Epidemiological Study. Adv. Exp. Med. Biol. 212, 251–263. 10.1007/978-1-4684-8240-9_32 - DOI - PubMed
    1. Desmyter J., LeDuc J. W., Johnson K. M., Brasseur F., Deckers C., van Ypersele de Strihou C. (1983). Laboratory Rat Associated Outbreak of Haemorrhagic Fever With Renal Syndrome Due to Hantaan-Like Virus in Belgium. Lancet 2 (8365-66), 1445–1448. 10.1016/s0140-6736(83)90797-3 - DOI - PubMed

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