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. 2021 Nov;101(3):115468.
doi: 10.1016/j.diagmicrobio.2021.115468. Epub 2021 Jul 5.

SARS-CoV-2 N gene mutations impact detection by clinical molecular diagnostics: reports in two cities in the United States

Mindy Leelawong  1 , Stephanie L Mitchell  2 , Randal C Fowler  1 , Edimarlyn Gonzalez  1 , Scott Hughes  1 , Marissa P Griffith  3 , Jane W Marsh  3 , Lee H Harrison  3 , Jennifer L Rakeman  4
Affiliations

SARS-CoV-2 N gene mutations impact detection by clinical molecular diagnostics: reports in two cities in the United States

Mindy Leelawong et al. Diagn Microbiol Infect Dis. 2021 Nov.

Abstract

Nasal and nasopharyngeal swab specimens tested by the Cepheid Xpert Xpress SARS-CoV-2 were analyzed by whole-genome sequencing based on impaired detection of the N2 target. Each viral genome had at least one mutation in the N gene, which likely arose independently in the New York City and Pittsburgh study sites.

Keywords: COVID-19; Cepheid XpertXpress; SARS-CoV-2; SARS-CoV-2 diagnostic testing; single nucleotide polymorphisms.

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Figures

Fig 1
Fig. 1
Relatedness of the N2-mutant genomes (A) SNP- based phylogenetic tree with each lineage represented by colored branches. (B) Heat map table of the pairwise SNP differences.
See this image and copyright information in PMC

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