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. 1987 Dec;368(12):1619-26.
doi: 10.1515/bchm3.1987.368.2.1619.

The major protamine from stallion sperm. Isolation and amino-acid sequence

H Ammer  1 , A Henschen
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The major protamine from stallion sperm. Isolation and amino-acid sequence

H Ammer et al. Biol Chem Hoppe Seyler. 1987 Dec.

Abstract

The major stallion protamine was isolated from sperm cell nuclei by extraction with 6M guanidine/5% mercaptoethanol, alkylation with 4-vinylpyridine and subsequent reversed-phase high-performance liquid chromatography. The primary structure of stallion protamine was determined by N-terminal sequencing of the intact protein and of the fragments obtained from thermolysin cleavage of the S-pyridylethylated and from endoproteinase Lys-C cleavage of the S-aminoethylated protein. Stallion protamine consists of 49 amino-acid residues and shows 49% identity with all other sequenced mammalian type 1 protamines.

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