Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Aug 24;32(9):107.
doi: 10.1007/s10856-021-06587-7.

Bone induction and defect repair by true bone ceramics incorporated with rhBMP-2 and Sr

Chunli Zhang #  1   2 , Gang Xu #  3   4 , Liwei Han  1   2 , Xiantong Hu  1   2 , Yantao Zhao  5   6 , Zhonghai Li  7   8
Affiliations

Bone induction and defect repair by true bone ceramics incorporated with rhBMP-2 and Sr

Chunli Zhang et al. J Mater Sci Mater Med. .

Abstract

Objective: To study the bone induction and defect repair of true bone ceramics (TBC) combined with rhBMP-2 and Sr.

Methods: MC3T3-E1 cells were used to evaluate the bioactivity of the composite. Cell proliferation activity was detected by CCK-8, ALP activity was detected by p-nitrophenyl phosphate (PNPP), and the differences of material surface topography were observed by scanning electron microscopy (SEM). Bone induction was verified by the implantation in nude mice. The rabbit femoral condyle defect model was achieved to verify the bone defect repair ability of the material.

Results: SEM results showed nearly the same surface morphology and cell proliferation quantified by CCK-8 showed that compared with TBC, both TBC&Sr and TBC&BMP-2&Sr had a significant promoting effect (P < 0.05). ALP activity result showed that the ALP activity of TBC&BMP-2&Sr was significantly higher than that of TBC alone (P < 0.05). The bone induction result showed that TBC&Sr had a small amount of new bone formation, and the new bone area was only 2.5 ± 0.11%. The bone induction activity of TBC&BMP-2&Sr was the highest, the new bone area was up to 75.36 ± 4.21%. Histological result of bone defect repair showed that TBC&BMP-2&Sr was also the highest, the new bone area was up to 72.42 ± 3.14%. The repair effect of TBC& BMP-2 was second, and better than that of TBC&Sr.

Conclusion: TBC combined with rhBMP-2 and Sr had the good bioactivity, obvious bone conduction and bone defect repair performance, laying the foundation of clinical application potentially.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
A The surface topography of the bone material observed by scanning electron microscopy (SEM) (100×); B Effects of four materials on the MC3T3-E1 proliferation (n = 4); C Effects of four materials on the ALP activity (n = 4); compared with TBC, *p < 0.05; compared with TBC&BMP-2, #p < 0.05
Fig. 2
Fig. 2
A Four weeks after the operation, the histology of each group was observed (HE staining, ×200); B Quantitative area of new bone formation (n = 3); compared with TBC&Sr, *p < 0.05; compared with TBC&BMP-2, #p < 0.05
Fig. 3
Fig. 3
Rabbit femoral condyle bone repair experiment. A The appearance of experimental material; B The repaired bone 6 weeks after the surgery; C Hard tissue section (HE staining, 40×); D Paraffin section (HE staining, 100×); E Paraffin section (TB staining, 100×); F Quantitative results of the histological sections (n = 3); compared with TBC&Sr, *p < 0.05; compared with TBC&BMP-2, #p < 0.05
See this image and copyright information in PMC

References

    1. Hasan A, Byambaa B, Morshed M, Cheikh MI, Shakoor RA, Mustafy T, et al. Advances in osteobiologic materials for bone substitutes. J Tissue Eng Regen Med. 2018;12:1448–68. doi: 10.1002/term.2677. - DOI - PubMed
    1. Kim YJ, Saiki CET, Silva K, Massuda CKM, de Souza Faloni AP, Braz-Silva PH, et al. Bone Formation in Grafts with Bio-Oss and Autogenous Bone at Different Proportions in Rabbit Calvaria. Int J Dent. 2020;2020:2494128.. doi: 10.1155/2020/2494128. - DOI - PMC - PubMed
    1. Qiao W, Ren X, Shi H, Li J, Yang T, Ma S, et al. [Biocompatibility research of true bone ceramics]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2017;31:1250–5. Chinese. - PMC - PubMed
    1. Ge M, Ge K, Gao F, Yan W, Liu H, Xue L, et al. Biomimetic mineralized strontium-doped hydroxyapatite on porous poly (L-lactic acid) scaffolds for bone defect repair. Int J Nanomed. 2018;13:1707–21. doi: 10.2147/IJN.S154605. - DOI - PMC - PubMed
    1. Luo X, Barbieri D, Duan R, Yuan H, Bruijn JD. Strontium-containing apatite/polylactide composites enhance bone formation in osteopenic rabbits. Acta Biomaterialia. 2015;26:331–7. doi: 10.1016/j.actbio.2015.07.044. - DOI - PubMed

MeSH terms