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. 2021 Aug 9:27:13-16.
doi: 10.1016/j.jor.2021.08.004. eCollection 2021 Sep-Oct.

Reamer-irrigator-aspirate versus bone marrow aspirate concentrate for osteoprogenitor cell retention and osteoinductive protein release on cancellous bone

Jane Liu  1   2 , Brett D Crist  1   2 , Aaron M Stoker  1   2 , James P Stannard  1   2 , James L Cook  1   2
Affiliations

Reamer-irrigator-aspirate versus bone marrow aspirate concentrate for osteoprogenitor cell retention and osteoinductive protein release on cancellous bone

Jane Liu et al. J Orthop. .

Abstract

Bone defects often require operative intervention with the use of bone graft. Two sources of autologous bone graft include reamer-irrigator-aspirate (RIA) and bone marrow aspirate concentrate (BMC). Osteoprogenitor cells and osteoconductive proteins have been identified in both sources. This study collected samples of these cells and proteins from a canine model and cultured them on human cancellous allograft bone blocks. Findings suggest that BMC may be preferred for indications that allow for delivery via injection, saturation of the patient's tissues, or an implanted scaffold, whereas RIA may be preferred when the biologic augment is delivered as a scaffold or graft.

Keywords: Autologous bone graft; Bone healing; Bone marrow aspirate concentrate; Pre-clinical animal model; Reamer-irrigator-aspirate.

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Conflict of interest statement

This study received grant funding from the AO Trauma North America Fellows Research program. Brett D. Crist, MD, and James P. Stannard, MD, are both paid consultants for DePuy Synthes; the Reamer-Irrigator-Aspirator (RIA) system used in this study is a DePuy Synthes product. The authors also received in-kind support from DePuy Synthes for the RIA products used in this study.

Figures

Fig. 1
Fig. 1
Representative 4X fluorescent viability staining (green) and phase contrast images of cells adherent to the bone block (cell viability) or well surface (phase contrast) after treatment with bone marrow concentrate (BMC) or reamer-irrigator-aspirate (RIA) bone material and cultured for 7 or 14 days. Images from the surface and interior of the bone block.
Fig. 2
Fig. 2
Media concentrations of dickkopf-related protein 1 (DKK1), epidermal growth factor (EGF), osteoprotegerin (OPG), osteopontin (OPN), and parathyroid hormone (PTH) from bone blocks treated with bone marrow concentrate (BMC) or reamer-irrigator-aspirate (RIA) on days 3, 7, and 14 of culture. (*) Significantly higher than other group on that day of culture.
See this image and copyright information in PMC

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