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. 2021 Feb 19:8:101283.
doi: 10.1016/j.mex.2021.101283. eCollection 2021.

Protocols for assessing antibacterial and water coagulation potential of Moringa oleifera seed powder

Affiliations

Protocols for assessing antibacterial and water coagulation potential of Moringa oleifera seed powder

George Aboagye et al. MethodsX. .

Abstract

This research evaluated Moringa oleifera seed powder (MOSP) as an antibacterial agent, and a coagulant. In the former, clinical isolates of Salmonella sp., Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) were used, and in the latter, river and stream water were used. Both the isolates and water samples were treated with MOSP at varying final concentrations of 0.001, 0.002, 0.004 and 0.017 g/ml. For the antibacterial assay, a dose of 0.017 g/ml of MOSP was effective on all three isolates with CFU/ml reduction of 99.4, 78.8 and 57.3% on Salmonella sp., E. coli and S. aureus respectively. An ANOVA confirmed this finding at P<0.05; 0.0014 between the treated and control samples. The water treatment assay also showed a reduction of total hardness, fluoride, phosphate, nitrate, total iron and manganese levels below the water quality standards. The MOSP could serve as a cost-effective product for process integration in raw water treatment systems in rural and urban settings. The study shows bioactivity of the seed powder of Moringa, and provides grounds to isolate the active component for commercialization and usage by the wider population with limited or no access to potable water.

Keywords: Antibacterial; Moringa oleifera seed powder; Potable water; Water coagulant.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image, graphical abstract
Graphical abstract
Fig. 1
Fig. 1
Location of river Tordze.
Fig. 2
Fig. 2
Effect of MOSP on S. aureus.
Fig. 3
Fig. 3
Effect of MOSP on E. coli.
Fig. 4
Fig. 4
Effect of MOSP on Salmonella sp.
Fig. 5
Fig. 5
Bactericidal effect of MOSP on isolates.

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