Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Aug 11:8:101487.
doi: 10.1016/j.mex.2021.101487. eCollection 2021.

The method of establishing the authenticity and quality of Hypericum perforatum L. and Salvia officinalis L

Zaual Temerdashev  1 , Victoria Milevskaya  1 , Elena Vinitskaya  1
Affiliations

The method of establishing the authenticity and quality of Hypericum perforatum L. and Salvia officinalis L

Zaual Temerdashev et al. MethodsX. .

Abstract

The paper presents methods for the criteria for quality control, authenticity and stability of preparations and raw materials based on Hypericum perforatum L. and Salvia officinalis L. Various methods of extracting the most valuable components that make up the studied plants, as well as methods for their chromatographic determination, were proposed, metrological characteristics were obtained.•The criteria for quality control and stability of drugs and raw materials were substantiated•The ratio of rutin to hyperforin determines the quality of Hypericum perforatum L.•The ratio of carnosic acid to carnosol characterizes Salvia officinalis L.

Keywords: Authenticity; Carnosic acid; Carnosol; HPLC-DAD-MS; Hyperforin; Hypericum perforatum L.; Rutin; Salvia officinalis L.; quality.

PubMed Disclaimer

Figures

Image, graphical abstract
Graphical abstract
Fig 1
Fig. 1
Chromatogram of Hypericum perforatum L. extract obtained by HPLC-DAD (Separation system 1): 1 – Protocatechuic acid; 2 – Neochlorogenic acid; 3 – Chlorogenic acid; 4 – (-)-epicatechin; 5 – Rutin; 6 – Hyperoside; 7 – Isoquercitrin; 8 – Quercitrin; 9 – Quercetin; 10 – I3,II8-biapigenin.
Fig 2
Fig. 2
Chromatogram of Hypericum perforatum L. extract obtained by HPLC-DAD (Separation system 2): 1 – Furohyperforin; 2 – Hyperforin; 3 – Adhyperforin; 4 – Pseudohypericin; 5 – Hypericin.
Fig 3
Fig. 3
Chromatogram of Salvia officinalis L. extract obtained by HPLC-DAD: 1 – Quinic acid; 2 – 3,4-dihydroxyphenyllactic acid; 3 – Protocatechuic acid; 4 – Chicoric acid; 5 – Protocatechuic aldehyde; 6 – Caffeic acid; 7 – Luteolin-7-O-β-D-rutinoside; 8 – Luteolin-7-O-β-D-glucoside; 9 – Luteolin-7-O-β-D-glucuronide; 10 – Rosmarinic acid; 11 – Apigenin 7-glucoside; 12 – Luteolin; 13 – Apigenin; 14 – Carnosol; 15 – Carnosic acid; 16 – Methyl carnosate.
See this image and copyright information in PMC

References

    1. Temerdashev Z., Milevskaya V., Shpigun O., Prasad S., Vinitskaya E., Ryaboko L. Stability of some biologically active substances in extracts and preparations based on St. John's Wort (Hypericum perforatum L.) and sage (Salvia officinalis L.) Ind. Crops Prod. 2020;156 doi: 10.1016/j.indcrop.2020.112879. - DOI
    1. Apaydin E.A., Maher A.R., Shanman R., Booth M.S., Miles J.N.V., Sorbero M.E., Hempel S. A systematic review of St. John's wort for major depressive disorder. Syst. Rev. 2016;5:148. doi: 10.1186/s13643-016-0325-2. - DOI - PMC - PubMed
    1. Barnes J., Anderson L.A., Phillipson J.D. St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J. Pharm. Pharmacol. 2001;53:583–600. doi: 10.1211/0022357011775910. - DOI - PubMed
    1. Bach-Rojecky L., Kalodera Z., Samarzija I. The antidepressant activity of Hypericum perforatum L. measured by two experimental methods on mice. Acta Pharm. 2004;54:157–162. - PubMed
    1. Crupi R., Mazzon E., Marino A., Spada G.La, Bramanti P., Battaglia F., Cuzzocrea S., Spina E. Hypericum perforatum treatment: effect on behaviour and neurogenesis in a chronic stress model in mice. BMC Complement. Altern. Med. 2011;11:1–10. doi: 10.1186/1472-6882-11-7. - DOI - PMC - PubMed

LinkOut - more resources