Effects of High-Frequency Electrical Stunning Current Intensities on Pre-Slaughter Stunning Stress and Meat Lipid Oxidation in Geese

Abstract

Intensive slaughtering with electrical stunning (ES) is replacing traditional manual slaughtering of geese in China. This study aimed to assess stunning stress and meat lipid oxidation in geese stunned by high-frequency current intensities. Forty male Yangzhou geese, 92 days old, were randomly allocated into five treatments with eight replicates per treatment. The geese in the control group were not stunned, while the other birds were stunned by alternating current (AC) in a water bath. Each bird received a current intensity of 20 mA (E20mA), 40 mA (E40mA), 70 mA (E70mA), or 100 mA (E100mA) for 10 s at 500 Hz. The gene expression of c-jun N-terminal kinase 1 tended to decrease in the E40mA birds (p = 0.08). Stunning with 40 mA resulted in the maximum serum uric acid and urea among the ES groups and decreased serum adrenocorticotropin and creatine kinase (p < 0.01) compared with 70 mA and 100 mA. Increasing the current intensity reduced the diphenylpicrylhydrazyl free radical elimination ability and total-superoxide dismutase linearly in goose breast meat at d 2 and in thigh meat at d 0 (all p < 0.01). Stunning geese with 40 mA at 500 Hz for 10 s could alleviate stunning stress and meat lipid oxidation.

Keywords: DPPH·EA; adrenocorticotropic hormone; electrical stunning; goose; hypothalamus-pituitary-adrenal axis; malondialdehyde; stunning stress; superoxide dismutase.

Figure 1

Effects of tissue and storage time on malondialdehyde (MDA) concentration. Means with no common superscripts between storage times (a, b) or tissues (X, Y) differ significantly (all p < 0.01).

Figure 2

Effects of tissue and storage time on total-superoxide dismutase (T-SOD) activity. Means with no common superscripts between storage times (a, b) or tissues (X, Y) differ significantly (all p < 0.01).