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. 2021 Aug 4;10(8):1603.
doi: 10.3390/plants10081603.

Effect of Propolis Paste and Mouthwash Formulation on Healing after Teeth Extraction in Periodontal Disease

Maria Jesús Lisbona-González  1 , Esther Muñoz-Soto  1 , Cristina Lisbona-González  1 , Marta Vallecillo-Rivas  1 , Javier Diaz-Castro  2   3   4 , Jorge Moreno-Fernandez  2   3
Affiliations

Effect of Propolis Paste and Mouthwash Formulation on Healing after Teeth Extraction in Periodontal Disease

Maria Jesús Lisbona-González et al. Plants (Basel). .

Abstract

This study investigated the antimicrobial effects of a mouthwash containing propolis and the effect of a propolis paste formulation on dental healing after teeth extraction in patients with periodontal disease. In the mouthwash experience, the population comprised 40 patients, which were divided as follows: the control mouthwash, 0.2% chlorhexidine (v/v) mouthwash, 2% (w/v) propolis mouthwash, and propolis + chlorhexidine mouthwash. The study of the propolis paste comprised a population of 60 patients with periodontal disease, and a total of 120 symmetric tooth extractions were performed. Propolis showed antimicrobial activity by itself, and especially with the chlorhexidine association. Three days after surgery in the teeth treated with control paste, only 13.4% had completely healed; however, with propolis paste, in 90% of the periodontal sockets, healing was complete. In addition, a reduction in Streptococci mutans and Lactobacilli cfu was observed with propolis, and especially with the association of chlorhexidine + propolis. Propolis mouthwash reduced bacterial proliferation, especially in association with chlorhexidine. Propolis paste is a viable alternative for socket healing after dental extraction. The knowledge gained from these findings will provide a foundation for similar propolis therapies in order to improve the healing process after dental surgery.

Keywords: chlorhexidine; oral healing; periodontal disease; propolis; teeth extraction.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Flowchart of the toothpaste assay.
Figure 2
Figure 2
Flowchart of the mouthwash assay.
Figure 3
Figure 3
Study design. Visit protocol on mouthwash experience. The registered participants were 40 patients who had signed the informed consent and understood the procedure. They were randomly divided into four groups and were instructed to use mouthwash three times a day for two days. Sample of saliva and CRT bacteria were performed just before intervention, on the same day, and two days later.
Figure 4
Figure 4
Study design. Visit protocol on healing process. The registered participants were 60 chronic periodontitis patients who had two or more teeth with high dental mobility, and they would need to have the extraction performed. Patients received test or control paste depending on randomization and were instructed to use it three times a day for one week on the dental socket after extraction.
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