Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase
- PMID: 34453707
- DOI: 10.1007/978-1-0716-1346-7_2
Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase
Abstract
Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating proteins. Its fast nucleotide exchange rate and inability to bind GDI in cytosol make it a unique and poorly understood Rab. Because the conventional, "GTP-locked" Rab mutation does not have the desired effect in Rab29, we present here the use of a fluorescence-based assay to characterize novel Rab29 mutants (I64T and V156G) that display faster nucleotide exchange rates, allowing for GEF-independent Rab29 activation.
Keywords: Fast exchange mutant; MANT-GDP; Nucleotide exchange; Rab GTPase; Rab29.
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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