A review on co-culturing of microalgae: A greener strategy towards sustainable biofuels production

Abstract

There is a growing global recognition that microalgae-based biofuel are environment-friendly and economically feasible options because they incur several advantages over traditional fossil fuels. Also, the microalgae can be manipulated for extraction of value-added compounds such as lipids (triacylglycerols), carbohydrates, polyunsaturated fatty acids, proteins, pigments, antioxidants, various antimicrobial compounds, etc. Recently, there is an increasing focus on the co-cultivation practices of microalgae with other microorganisms to enhance biomass and lipid productivity. In a co-cultivation strategy, microalgae grow symbiotically with other heterotrophic microbes such as bacteria, yeast, fungi, and other algae/microalgae. They exchange nutrients and metabolites; this helps to increase the productivity, therefore facilitating the commercialization of microalgal-based fuel. Co-cultivation also facilitates biomass harvesting and waste valorization, thereby help to build an algal biorefinery platform for bioenergy production along with multivariate high value bioproducts and simultaneous waste bioremediation. This article comprehensively reviews various microalgae cultivation practices utilizing co-culture approaches with other algae, fungi, bacteria, and yeast. The review mainly focuses on the impact of several binary culture strategies on biomass and lipid yield. The advantages and challenges associated with the procedure along with their respective cultivation modes have also been presented and discussed in detail.

Keywords: Biomass; Co-cultivation; Lipid; Microalgae-bacteria; Microalgae-fungi; Microalgae-yeast.