Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec;476(12):4343-4349.
doi: 10.1007/s11010-021-04237-8. Epub 2021 Aug 28.

Cardiac dysfunction in spontaneously hypertensive old rats is associated with a significant decrease of SUR2 expression

Ruslan B Strutynskyi  1 , Serhii V Goncharov  1 , Lesya V Tumanovska  1 , Vasyl S Nagibin  2 , Victor E Dosenko  1
Affiliations

Cardiac dysfunction in spontaneously hypertensive old rats is associated with a significant decrease of SUR2 expression

Ruslan B Strutynskyi et al. Mol Cell Biochem. 2021 Dec.

Abstract

ATP-sensitive potassium (KATP) channels are participants of mechanisms of pathological myocardial remodeling containment. The aim of our work was to find the association of changes in the expression of Kir6.1, Kir6.2, SUR1, and SUR2 subunits of KATP channels with changes in heart function and structure during aging under conditions of the constant increase of vascular pressure. The experiments were carried out on young and old spontaneously hypertensive rats (SHR) and Wistar rats. The expression levels of KATP channels subunits were determined using reverse transcription and quantitative PCR. It is shown that the mRNA expression level of Kir6.1 in young SHR rats is significantly lower (6.3-fold, p = 0.035) than that of young Wistar rats that may be one of the causes of arterial hypertension in SHR. At the same time, mRNA expression of both Kir6.1 and Kir6.2 in old SHR rats was significantly higher (6.8-fold, p = 0.003, and 5.9-fold, p = 0.006, respectively) than in young hypertensive animals. In both groups of old animals, SUR2 expression was significantly reduced compared to young animals, in Wistar rats at 3.87-fold (p = 0.028) and in SHR rats at 48.2-fold (p = 0.033). Changes in SUR1 expression were not significant. Thus, significant changes in the cardiovascular system, including impaired function and structure of the heart in old SHR rats, were associated with a significant decrease in SUR2 expression that may be one of the mechanisms of heart failure decompensation. Therefore, it can be assumed that increased expression of SUR2 may be one of the protective mechanisms against pathological myocardial remodeling.

Keywords: Aging; Expression of KATP channels; Hypertension; Myocardial remodeling; SUR2.

PubMed Disclaimer

References

    1. Nichols CG (2006) KATP channels as molecular sensors of cellular metabolism. Nature 440(7083):470–476 - DOI
    1. Olson TM, Terzic A (2010) Human KATP channelopathies: diseases of metabolic homeostasis. Pflugers Arch 460(2):295–306 - DOI
    1. Du Q, Jovanović S, Clelland A, Sukhodub A, Budas G, Phelan K et al (2006) Overexpression of SUR2A generates a cardiac phenotype resistant to ischemia. FASEB J 20(8):1131–1141 - DOI
    1. Crawford RM, Jovanović S, Budas GR, Davies AM, Lad H, Wenger RH et al (2003) Chronic mild hypoxia protects heart-derived H9c2 cells against acute hypoxia/reoxygenation by regulating expression of the SUR2A subunit of the ATP-sensitive K+ channel. J Biol Chem 278(33):31444–31455 - DOI
    1. Mohammed Abdul KS, Jovanović S, Sukhodub A, Du Q, Jovanović A (2014) Upregulation of cardioprotective SUR2A by sub-hypoxic drop in oxygen. Biochim Biophys Acta 1843(11):2424–2431 - DOI

LinkOut - more resources