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. 2017 Mar 5;7(5):e2153.
doi: 10.21769/BioProtoc.2153.

Olfactory Avoidance Test (Mouse)

Hiroo Takahashi  1 , Akio Tsuboi  1
Affiliations

Olfactory Avoidance Test (Mouse)

Hiroo Takahashi et al. Bio Protoc. .

Abstract

In mice, olfaction plays a pivotal role in the various behaviors, such as feeding, mating, nursing and avoidance. Behavioral tests that analyze abilities of odor detection and recognition using genetically modified mice reveal the contribution of target genes to the olfactory processing. Here, we describe the olfactory avoidance test to investigate the odor detection ability in mice.

Keywords: Avoidance; Behavioral test; Freezing; Mouse; Odor detection threshold; Olfaction.

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Figures

Figure 1.
Figure 1.. Olfactory avoidance test.
A. Schema of the experimental procedure; B. Apparatus of the olfactory avoidance test.
Figure 2.
Figure 2.. Sample data of the olfactory avoidance test.
A and B. Freezing time (A) and avoidance index (B) in wild-type mice; C. When a lower amount (4 μl) of nTMT was employed, 5T4 knockout mice remarkably reduced the freezing time, compared with wild-type mice (see the detail in Takahashi et al., 2016 ).
Video 1.
Video 1.. Freezing response in wild-type mice for 40 μl nTMT
See this image and copyright information in PMC

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