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. 2017 Mar 5;7(5):e2154.
doi: 10.21769/BioProtoc.2154.

Olfactory Habituation-dishabituation Test (Mouse)

Hiroo Takahashi  1 , Akio Tsuboi  1
Affiliations

Olfactory Habituation-dishabituation Test (Mouse)

Hiroo Takahashi et al. Bio Protoc. .

Abstract

Olfaction plays a fundamental role in the various behaviors such as feeding, mating, nursing, and avoidance in mice. Behavioral tests that characterize abilities of odor detection and recognition using genetically modified mice reveal the contribution of target genes to the olfactory processing. Here, we describe the olfactory habituation-dishabituation test for investigating the odor detection threshold in mice.

Keywords: Behavioral test; Mouse; Odor detection threshold; Olfaction; Olfactometer.

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Figures

Figure 1.
Figure 1.. Apparatus of the olfactory habituation-dishabituation test
Figure 2.
Figure 2.. Schema of the experimental procedure.
A. Schema of the experimental procedure; B. Investigation time is defined as the time when the nose entered the 2.5-cm square area near the gas port (blue dotted line).
Figure 3.
Figure 3.. Sample data of the habituation-dishabituation test.
A. Investigation time in the 1st and 2nd trials using 6.3 μM eugenol in wild-type mice. B. Differences in investigation times between the 1st and 2nd trials using different concentrations of eugenol in wild-type mice and 5T4 knockout (KO) mice, in which the 5T4 coding region was replaced by LacZ ( Southgate et al., 2010 ). A single-pass transmembrane glycoprotein, 5T4, is known to regulate the dendritic development of olfactory bulb interneurons. At 6.3 μM, the investigation time of 5T4 KO mice was remarkably shorter than that of wild-type mice. These results suggest that a subset of olfactory bulb interneurons is required for the odor detection behavior (see the detail in Takahashi et al., 2016 ).
Video 1.
Video 1.. Response of wild-type mice to 6.3 μM eugenol
See this image and copyright information in PMC

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