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Comment
. 2021 Nov;28(11):3193-3195.
doi: 10.1038/s41418-021-00863-3. Epub 2021 Aug 31.

Mapping the hotspots for DNA repair synthesis in human brain organoids

Tomohisa Kato Jr  1 , Alysson R Muotri  2   3   4
Affiliations
Comment

Mapping the hotspots for DNA repair synthesis in human brain organoids

Tomohisa Kato Jr et al. Cell Death Differ. 2021 Nov.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Future directions of research following the reports of Reid et al. and Wu et al.
(Flow to the right side) In addition to NEUROD1-induced neurons, other types of neurons might be analyzed. (Flow to the left side) Region-specific brain organoids are generated and analyzed. By modulating the length of the culture period, brain organoids have been shown to reach postnatal stages. With genetic engineering technology, brain organoids may mimic later stages of development. Both sides of studies may use iPSCs of healthy individuals and those of patients with DNA Repair deficiencies or isogenic lines mimicking the mutation of patients generated by gene-editing technology.
See this image and copyright information in PMC

Comment on

  • Neuronal enhancers are hotspots for DNA single-strand break repair.
    Wu W, Kargbo-Hill SE, Nathan WJ, Paiano J, Callen E, Wang D, Shinoda K, van Wietmarschen N, Colón-Mercado JM, Zong D, De Pace R, Shih HY, Coon S, Parsadanian M, Pavani R, Hanzlikova H, Park S, Jung SK, McHugh PJ, Canela A, Chen C, Casellas R, Caldecott KW, Ward ME, Nussenzweig A. Wu W, et al. Nature. 2021 May;593(7859):440-444. doi: 10.1038/s41586-021-03468-5. Epub 2021 Mar 25. Nature. 2021. PMID: 33767446 Free PMC article.
  • Incorporation of a nucleoside analog maps genome repair sites in postmitotic human neurons.
    Reid DA, Reed PJ, Schlachetzki JCM, Nitulescu II, Chou G, Tsui EC, Jones JR, Chandran S, Lu AT, McClain CA, Ooi JH, Wang TW, Lana AJ, Linker SB, Ricciardulli AS, Lau S, Schafer ST, Horvath S, Dixon JR, Hah N, Glass CK, Gage FH. Reid DA, et al. Science. 2021 Apr 2;372(6537):91-94. doi: 10.1126/science.abb9032. Science. 2021. PMID: 33795458 Free PMC article.

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