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. 2021 Sep;28(9):5349-5358.
doi: 10.1016/j.sjbs.2021.05.064. Epub 2021 May 29.

Chemical characteristics and targeted encapsulated Cordia myxa fruits extracts nanoparticles for antioxidant and cytotoxicity potentials

Affiliations

Chemical characteristics and targeted encapsulated Cordia myxa fruits extracts nanoparticles for antioxidant and cytotoxicity potentials

Khaled F El-Massry et al. Saudi J Biol Sci. 2021 Sep.

Abstract

A promising Cordia myxa fruit (CMF) extract targets an additional incorporation in functional foods. It retains appropriate health welfares owing to its antioxidant properties with limited incorporation in food matrices due its hydrophobicity. Therefore, CMF extract micro- and nanocapsulation was performed to protect and facilitate consistency of produced hydrophobic foods matrices. Furthermore, to determine its phytochemicals, antioxidant, and cytotoxic effects by applying analytical HPLC, FRAP and SRB assay, respectively. HPLC analysis of the tested extracts revealed the presence of, 25.59 ± 1.78 mg catechin/g, 69.68 ± 4.20 mg quercetin/g, and 112.72 ± 8.38 mg gallic acid/g extract. The CMF extract displayed a potent DPPH radicals' scavenger and FRAP high reduction capability in a dose-dependent manner. The potent pharmacological activities of CMF extract may be ascribed to high concentration of polyphenolics including flavonoids which strongly reported to possess an antitumor and antioxidant activities. To confirm the efficient CMF incorporation in micro- and nanosystems and their thermal stabilities to withstand the high temperatures applied during some food processing. DSC of the apparent melt of non-capsulated CMF and encapsulated forms (MCMF and NCMF) in sodium alginate gel and beads was studied. Results showed that melting point of CMF extract (86.17 °C) indicating its inability whereas the MCMF and NCMF melting points (226.45 and 383.87 °C, respectively) demonstrating the capability of expending alginate - packaging material to shield the vital active compounds of C. myxa fruit to be applied in different targeted delivery products especially that disclosed to high thermal treatments.

Keywords: Antioxidant; Cordia myxa fruit; Cytotoxicity; Encapsulation; Flavonoids; Phenolic content.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
The surface morphology and shape of the CMF encapsulated extracts (micro- and nanoencapsules) examined by TEM.
Fig. 2
Fig. 2
Encapsulation Efficiency (%) of micro- and nanocapsulated CMFE forms.
Fig. 3
Fig. 3
Thermogram obtained by DSC for CMF uncapsulated extract.
Fig. 4
Fig. 4
Thermogram obtained by DSC for CMF microcapsulated extract.
Fig. 5
Fig. 5
Thermogram obtained by DSC for CMF nanocapsulated extract.
Fig. 6
Fig. 6
Storage stability of CMF extract forms during storage at four °C.
Fig. 7
Fig. 7
FRAP of CMF (non-, micro- and nanocapsulated) extracts.
Fig. 8
Fig. 8
Cell viability percentage (posttreatment) of Hep G2: (A) CMF extract, (M) microcapsulated CMF extract, and (N) nanocapsulated CMF extract Cisplatin using SRB assay.
Fig. 9
Fig. 9
Cell viability percentage (posttreatment) of THLE2: (A) CMF extract, (M) microcapsulated CMF extract, and (B) nanocapsulated CMF extract Cisplatin using SRB assay.

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