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. 2021 Sep 1;224(12 Suppl 2):S204-S208.
doi: 10.1093/infdis/jiab056.

Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes

Mahamoudou Ouattara  1 , Mamadou Tamboura  2 , Dinanibè Kambiré  2 , Kim Anh Lê  3 , Thanh Van Phan  4 , Srinivasan Velusamy  1 , Hien Anh Nguyen  3 , Dai Vo Thi Trang  4 , Fernanda C Lessa  1 , Makiko Iijima  5 , Dac Trung Nguyen  3 , Stephanie B Schwartz  1 , Lesley McGee  1 , Rasmata Ouédraogo Traoré  2 , Bernard Beall  1
Affiliations

Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes

Mahamoudou Ouattara et al. J Infect Dis. .

Abstract

The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.

Keywords: Streptococcus pneumoniae; direct real-time PCR; pneumococcal serotype.

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Figures

Figure 1.
Figure 1.
Bland–Altman plot (A) and Krouwer plot (B) of the cycle threshold differences (ΔCt) between the direct real-time polymerase chain reaction (PCR) method and the traditional real-time PCR method. The upper and lower limits of agreement are indicated by the upper and lower solid lines (SD = standard deviation of ΔCt). The dashed line indicates the mean ΔCt and the dots represent ΔCt values. These data were generated using 120 clinical cerebrospinal fluid samples.
See this image and copyright information in PMC

References

    1. Weiser JN, Ferreira DM, Paton JC. Streptococcus pneumoniae: transmission, colonization and invasion. Nat Rev Microbiol 2018; 16:355–67. - PMC - PubMed
    1. Ganaie F, Saad JS, McGee L, et al. . A new pneumococcal capsule type, 10D, is the 100th serotype and has a large cps fragment from an oral Streptococcus. mBio 2020; 11:e00937-20. - PMC - PubMed
    1. Pimenta FC, Roundtree A, Soysal A, et al. . Sequential triplex real-time PCR assay for detecting 21 pneumococcal capsular serotypes that account for a high global disease burden. J Clin Microbiol 2013; 51:647–52. - PMC - PubMed
    1. Sakai F, Chochua S, Satzke C, et al. . Single-plex quantitative assays for the detection and quantification of most pneumococcal serotypes. PLoS One 2015; 10:e0121064. - PMC - PubMed
    1. Tarragó D, Fenoll A, Sánchez-Tatay D, et al. . Identification of pneumococcal serotypes from culture-negative clinical specimens by novel real-time PCR. Clin Microbiol Infect 2008; 14:828–34. - PubMed

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