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. 2022 Mar;67(1):330-339.
doi: 10.1007/s11686-021-00466-5. Epub 2021 Sep 3.

A New Species of Paradiplozoon (Monogenea: Diplozoidae), A Gill Parasite of the Schizothorax Fish (Cyprinidae: Schizothoracinae) from the Yarkand River, Xinjiang, China

Kadirden Arken #  1 , Cui-Lan Hao #  1 , Ai-Ming Guo  1 , Wen-Run Zhang  1 , Meng-Jie Rong  1 , Wuljan Kamal  1 , Sheng-Li Tian  1 , Munira Kadir  1 , Cheng Yue  2
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A New Species of Paradiplozoon (Monogenea: Diplozoidae), A Gill Parasite of the Schizothorax Fish (Cyprinidae: Schizothoracinae) from the Yarkand River, Xinjiang, China

Kadirden Arken et al. Acta Parasitol. 2022 Mar.

Abstract

Introduction: Monogeneans of the genus Paradiplozoon were found on the gills of specimens of five species of schizothoracid caught using fyke nets in the upper stream of the Yarkand River, Xinjiang, China in May-August 2019.

Methods: The preserved parasite were stained with boric acid magenta and hematoxylin, respectively. Morphological observations, line drawings, photomicrographs and measurements were made in Nikon ECLIPSE E200 imaging optical microscope and digitally edited. The molecular analysis included the study of the sequence of the second internal transcribed spacer (ITS 2) of the ribosomal DNA region, calculation and analysis of genetic distance, with phylogenetic reconstructions based on the Bayesian inference and Maximum Likelihood analysis.

Results: The natural infection rate of host fish was 10-88%. Morphological analysis indicated that the average length of the new species was 2.125 mm while the width was 0.69 mm. The anterior part was 1.387 mm in length and the average length of the posterior part was 0.545 mm. The vitellaria was well-developed and located in the front of the body. A single ovary (oval shaped) was located at the back end of the reproductive binding area. A testis (irregular mass) was located behind or parallelled to the ovary. The new species can be distinguished from all the recorded Paradiplozoon species in terms of morphological characteristics such as haptor, clamp and central hook morphology, intestine shape and body size. In addition, the second internal transcribed spacer (ITS 2) of the ribosomal DNA region of the diplozoid was compared with that of known diplozoids previously published. It indicated that there were significant differences between the new species and the published diplozoids.

Conclusion: Both morphological and molecular analysis support that the diplozoid is a new species. Based on the sampling location, the new species was named Paradiplozoon yarkandense n. sp.

Keywords: Monogenea; New species; Paradiplozoon; Schizothoracids.

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