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. 2021 Sep 4;20(1):61.
doi: 10.1186/s12941-021-00469-0.

Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity

Hadas Kon  1 , Shirin Abramov  1 , Sammy Frenk  1 , David Schwartz  1 , Ohad Shalom  2 , Amos Adler  2   3 , Yehuda Carmeli  1   3 , Jonathan Lellouche  4   5
Affiliations

Multiplex lateral flow immunochromatographic assay is an effective method to detect carbapenemases without risk of OXA-48-like cross reactivity

Hadas Kon et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: It is essential to detect carriers of carbapenemase-producing Enterobacterales in order to implement infection control measures. The objectives of this study was to evaluate the NG-Test® CARBA 5 (CARBA 5) assay for detection of five carbapenemases and to assess the cross reactivity of other OXA-type carbapenemases with the OXA-48-like specific antibodies.

Methods: A total of 197 Enterobacterales isolates were tested. To evaluate the cross reactivity, 73 carbapenem-resistant A. baumannii, harboring OXA-type variants, were tested. Polymerase chain reaction (PCR) served as gold standard for carbapenemase identification.

Results: Excellent agreement was found between PCR and CARBA 5, for all but one isolate. The single false positive result (a blaSME positive S. marcescens isolate) was incorrectly positive for blaOXA-48 by CARBA 5. No cross reactivity was observed. The sensitivity and specificity were 100.0% and 98.0%, respectively.

Conclusions: The CARBA 5 assay is highly sensitive and specific and is recommended as a tool for the detection of the main carbapenemases of interest in clinical microbiology laboratories.

Keywords: Carbapenem resistant; Carbapenemase; Cross reactivity; Enterobacterales; NG-Test® CARBA 5 assay; OXA-48-like.

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Conflict of interest statement

The authors declare that they have no competing interests.

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