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. 2021 Jun;13(2):156-161.
doi: 10.1055/s-0041-1730849. Epub 2021 Jun 19.

Pseudobasophilia as a Screening Tool in Dengue: A Single Center Study

Kevin Manuel  1 , Marie Moses Ambroise  1 , Anita Ramdas  1 , Renu G'Boy Varghese  1
Affiliations

Pseudobasophilia as a Screening Tool in Dengue: A Single Center Study

Kevin Manuel et al. J Lab Physicians. 2021 Jun.

Abstract

Objectives Proper serological testing for the definite diagnosis of dengue is costly and may not be easily available in a resource-limited setting. Hematological parameters can help in the early identification of dengue cases. This study aims to evaluate the occurrence and utility of pseudobasophilia in identifying dengue-affected patients. Materials and Methods This retrospective cross-sectional study included 1,304 dengue cases confirmed by serology and 1,044 dengue serology negative acute febrile illness cases as controls. Complete blood count (CBC) values of the first EDTA (ethylenediamine tetraacetic acid) blood sample from automated hematology analyzers were reviewed. The hematological parameters in the dengue and control groups were compared and the variation of these parameters with the day of fever was also analyzed. Statistical Analysis Mann-Whitney's test, Kruskal-Wallis test, and Fisher's exact test were used for statistical analysis. A p -value < 0.05 was considered statistically significant for all tests. Results There was a statistically significant variation between dengue cases and controls for hematocrit, platelet count, mean platelet volume, total white blood cell count, and absolute basophil count. The dengue group had a higher hematocrit from day 2 to day 10, platelet count ≤ 100,000/µL from day 4 to day 9, higher mean platelet volume from day 2 to day 7, leucopenia from day 3 to day 5, and higher absolute basophil count from day 2 to day 10. Interestingly, pseudobasophilia was seen in 533 (40.87%) of dengue cases and only 3 (0.28%) of the controls. Pseudobasophilia was also observed to have an increasing trend to the day of fever. Conclusion Pseudobasophilia along with other CBC parameters is useful and cost effective for the early identification of dengue. This can prompt early investigations and supportive treatment leading to improved clinical outcomes.

Keywords: automated hematology; dengue; fever; lymphocytes.

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Conflict of interest statement

Conflicts of InterestAuthors’ ContributionsInstitutional Review Board Approval No conflicts of interest. K.M. contributed toward the conception of idea, research design, data collection, statistical analysis, and report writing. M.M.A. did the research design, data collection, statistical analysis, report writing, and critical reviewing. A.R. and R.G.V. provided technical support and did report writing and critical reviewing. This retrospective study was conducted after obtaining approval from the Institutional Ethics Committee [IEC: RC/18/53]. Waiver of informed consent was granted for the study.

Figures

Fig. 1
Fig. 1
Hematological variations in the dengue and control groups according to the day of fever. ( A ) Hematocrit variation. ( B ) Platelet count variation. ( C ) Mean platelet volume variation. ( D ) White blood cell count variation. ( E ) Absolute basophil count variation.
See this image and copyright information in PMC

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