The identification and performance assessment of dominant bacterial species during linear alkylbenzene sulfonate (LAS)-biodegradation in a bioelectrochemical system

Abstract

The anionic surfactant linear alkylbenzene sulfonate (LAS) is a major chemical constituent of detergent formulation. Regarding the recalcitrant nature of sulfonoaromatic compounds, discharging these substances into wastewater collection systems is a real environmental issue. A study on LAS biodegradation based on bioelectrochemical treatment and in the form of developing a single-chamber microbial fuel cell with air cathode is reported in the present work. Pretreatment study showed LAS concentration of 60 ppm resulted in the highest anaerobic LAS removal of 57%; so, this concentration was chosen to run the MFC. After the sustained anodic biofilm was formed, LAS degradation rate during 4 days in MFC was roughly 76% higher than that in the serum bottle, which indicated the role of the bioelectrochemical process in improving anaerobic LAS removal. Additionally, through 16S rRNA gene sequencing, the dominant bacterial species in the biofilm was identified as Pseudomonas zhaodongensis NEAU-ST5-21(T) with about 98.9% phylogenetic similarity and then a pathway was proposed for LAS anaerobic biodegradation. The MFC characteristics were assessed by pH monitoring as well as scanning electron microscopy and current density evolution.

Keywords: 16s rRNA sequencing; Biodegradation; Bioelectrochemical system; Linear alkylbenzene sulfonate (LAS); Pseudomonas zhaodongensis.