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. 2022:2436:183-192.
doi: 10.1007/7651_2021_413.

Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask

Anastasiia Artuyants  1   2 , Vanessa Chang  1   2 , Gabrielle Reshef  1   2 , Cherie Blenkiron  1   3   4 , Lawrence W Chamley  1   2 , Euphemia Leung  4 , Colin L Hisey  5   6
Affiliations

Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask

Anastasiia Artuyants et al. Methods Mol Biol. 2022.

Abstract

The efficient production of extracellular vesicles (EVs) from adherent cells in vitro can be challenging when using conventional culture flasks. Issues such as low cell density leading to low EV yield, and the inability to completely remove bovine serum EVs without starvation contribute to this challenge. By comparison, the two-chamber CELLine adherent bioreactor can produce significantly more EVs with improved time, space, and resource efficiency. Furthermore, it is highly accessible and can continually produce EVs using long term cultures without the need for passaging. Lastly, the 10 kDa semipermeable, cellulose acetate membrane separating the cell and media chambers allows for the continual use of bovine serum in the media chamber while preventing bovine EVs from contaminating the conditioned media.

Keywords: CELLine bioreactor; Exosomes; Extracellular vesicles; Microvesicles.

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References

    1. Lehrich BM et al (2018) Fetal bovine serum-derived extracellular vesicles persist within vesicle-depleted culture media. Int J Mol Sci 19(11):3538 - DOI
    1. Shelke GV et al (2014) Importance of exosome depletion protocols to eliminate functional and RNA-containing extracellular vesicles from fetal bovine serum. J Extracell Vesicles 3
    1. Lehrich BM, Liang Y, Fiandaca MS (2021) Foetal bovine serum influence on in vitro extracellular vesicle analyses. J Extracell Vesicles 10(3):e12061 - DOI
    1. Yan IS, Shukla N, Borrelli DA, Patel T (2018) Use of a hollow fiber bioreactor to collect evs from cells in culture. In: Walker J (ed) Extracellular RNA: methods in molecular biology, vol 1740. Springer Protocols, New York
    1. Watson DC et al (2018) Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes. J Extracell Vesicles 7(1):1442088 - DOI

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