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. 2022 Jan;13(1):72-77.
doi: 10.1007/s13238-021-00874-3. Epub 2021 Sep 7.

Persisting lung pathogenesis and minimum residual virus in hamster after acute COVID-19

Lunzhi Yuan #  1 , Huachen Zhu #  2   3 , Ming Zhou #  1 , Jian Ma #  1 , Rirong Chen #  2   3 , Liuqin Yu  2   3 , Wenjia Chen  2   3 , Wenshan Hong  2   3 , Jia Wang  2   3 , Yao Chen  1 , Kun Wu  1 , Wangheng Hou  1 , Yali Zhang  1 , Shengxiang Ge  1 , Yixin Chen  1 , Quan Yuan  1 , Qiyi Tang  4 , Tong Cheng  5 , Yi Guan  6   7 , Ningshao Xia  8   9
Affiliations

Persisting lung pathogenesis and minimum residual virus in hamster after acute COVID-19

Lunzhi Yuan et al. Protein Cell. 2022 Jan.
No abstract available

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Figures

Figure 1
Figure 1
Acute infection and recovery of hamsters with SARS-CoV-2. (A) Schematic diagram of SARS-CoV-2 infection and animal operations for observation of persisting symptoms after acute SARS-CoV-2 infection. Hamsters were intranasally inoculated with 1 × 102 to 1 × 105 PFU of SARS-CoV-2, respectively (n = 3/group). All of the animals were euthanized at 42 dpi for virological and histological analysis. (B) Body weight changes after SARS-CoV-2 infection from 0 to 7 dpi (n = 3/group). (C) Titers of live virus in lung tissues collected at 42 dpi by a CPE-based titration assay in 96-well plates. For each hamster, one near-hilum lung tissue sample and one away-hilum lung tissue sample were used for titration. (D) The samples collected at 42 dpi were incubated with Vero-E6 cells in 24-well plates for a 7-day culture. (E) And then, the Vero-E6 cells were collected for a second-round titration. (F) Titers of serum neutralizing antibody levels from 0 to 42 dpi (n = 3/group). There is no significant difference between the NAb titers of different groups at 42 dpi (1 × 102 PFU vs. 1 × 103 PFU, P > 0.05; 1 × 102 PFU vs. 1 × 104 PFU, P > 0.05; 1 × 102 PFU vs. 1 × 105 PFU, P > 0.05). (G) Representative image of immunohistochemistry staining for SARS-CoV-2 NP in lung lobe sections collected at 42 dpi (left). The area in the scattered square was enlarged (bar = 100 μm), the arrows points to the NP positive cells
Figure 2
Figure 2
Pathological analysis of lung lobe tissues collected from hamsters in acute SARS-CoV-2 infection phase and convalescent phase. For each hamster, four lung lobes were fixed in formalin for pathological analysis. Representative H&E staining for lung lobe sections collected from SARS-CoV-2 infected hamsters at (A) 5, (B) 7 and (C) 42 dpi, respectively. These hamsters were infected with 1 × 102 PFU (line 1), 1 × 103 PFU (line 2), 1 × 102 PFU (line 3) and 1 × 105 PFU (line 4) of SARS-CoV-2, respectively. H&E staining for all the rest hamsters were shown in Fig. S3. Comprehensive pathological scores for lung of hamsters during (D) the acute SARS-CoV-2 infection phase and (E) the convalescent phase. Scores were determined based on the severity and percentage of injured areas for each lung lobe. There are significant differences between the lung pathological scores of different groups at 5 dpi (1 × 102 PFU vs. 1 × 103 PFU, P = 0.048; 1 × 102 PFU vs. 1 × 104 PFU, P > 0.05; 1 × 102 PFU vs. 1 × 105 PFU, P = 0.022). There is no significant difference between the lung pathological scores of different groups at 7 and 42 dpi (1 × 102 PFU vs. 1 × 103 PFU, P > 0.05; 1 × 102 PFU vs. 1 × 104 PFU, P > 0.05; 1 × 102 PFU vs. 1 × 105 PFU, P > 0.05). (F) The linear relationship between body weight change and average lung pathological score of each individual hamster
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