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Review
. 2021 Dec;118(12):4577-4589.
doi: 10.1002/bit.27935. Epub 2021 Sep 27.

Sortase A: A chemoenzymatic approach for the labeling of cell surfaces

Poonam Kumari  1 , Sujoy Bowmik  1 , Sudipto Kumar Paul  1 , Bidisha Biswas  1 , Sanjay K Banerjee  1 , Upadhyayula Surayanarayana Murty  1 , Velayutham Ravichandiran  2 , Utpal Mohan  2
Affiliations
Review

Sortase A: A chemoenzymatic approach for the labeling of cell surfaces

Poonam Kumari et al. Biotechnol Bioeng. 2021 Dec.

Abstract

Sortase A, a transpeptidase enzyme is present in many Gram-positive bacteria and helps in the recruitment of the cell surface proteins. Over the last two decades, Sortase A has become an attractive tool for performing in vivo and in vitro ligations. Sortase A-mediated ligation has continuously been used for its specificity, robustness, and highly efficient nature. These properties make it a popular choice among protein engineers as well as researchers from different fields. In this review, we give an overview of Sortase A-mediated ligation of various molecules on the cell surfaces, which can have diverse applications in interdisciplinary fields.

Keywords: S. aureus; Sortase A; enzyme; ligation.

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References

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