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. 2022 Feb;47(2):315-326.
doi: 10.1007/s11064-021-03444-5. Epub 2021 Sep 8.

The Role of Klotho Protein Against Sevoflurane-Induced Neuronal Injury

Wan-Yi Lian #  1 , Ze-Peng Lu #  1 , Wei Zhao  1 , Jia-Qi Zou  1 , Zi-Ying Lu  1 , Li-Bing Zhou  2 , Hong-Yi Lei  3
Affiliations

The Role of Klotho Protein Against Sevoflurane-Induced Neuronal Injury

Wan-Yi Lian et al. Neurochem Res. 2022 Feb.

Abstract

The effects of general anesthetics on the developing brain have aroused much attention in recent years. Sevoflurane, a commonly used inhalation anesthetic especially in pediatric anesthesia, can induce developmental neurotoxicity. In this study, the differentially expressed mRNAs in the hippocampus of newborn rats exposed to 3% sevoflurane for 6 h were detected by RNA-Sequencing. Those data indicated that the mRNA of Klotho was increased after exposure to sevoflurane. Moreover, the protein expression of Klotho was assayed by Western Blot. Besides over-expression and under-expression of Klotho protein, we also detected changes of cell proliferation, ROS, JC-1, and Bcl-2/Bax ratio in PC12 cells exposed to sevoflurane. After exposure to 3% sevoflurane, the expression of Klotho protein increased in the hippocampus of neonatal rats. In PC12 cells, exposure to sevoflurane could increase cellular ROS level, reduce mitochondrial membrane potential and Bcl-2/Bax ratio. While overexpression of Klotho alleviated the above changes, knockdown of Klotho aggravated the injury of sevoflurane. Klotho protein could reduce oxidative stress and mitochondrial injury induced by sevoflurane in the neuron.

Keywords: Apoptosis; Klotho; Neurotoxicity; Oxidative stress; Sevoflurane.

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References

    1. Jevtovic-Todorovic V, Hartman R, Izumi Y, Benshoff N, Dikranian K, Zorumski C, Olney J, Wozniak D (2003) Early exposure to common anesthetic agents causes widespread neurodegeneration in the developing rat brain and persistent learning deficits. J Neurosci 23(3):876–882. https://doi.org/10.1523/jneurosci.23-03-00876.2003 - DOI - PubMed - PMC
    1. Muramatsu R, Ikegaya Y, Matsuki N, Koyama R (2007) Neonatally born granule cells numerically dominate adult mice dentate gyrus. Neuroscience 148(3):593–598. https://doi.org/10.1016/j.neuroscience.2007.06.040 - DOI - PubMed
    1. Creeley C, Dikranian K, Dissen G, Back S, Olney J, Brambrink A (2014) Isoflurane-induced apoptosis of neurons and oligodendrocytes in the fetal rhesus macaque brain. Anesthesiology 120(3):626–638. https://doi.org/10.1097/aln.0000000000000037 - DOI - PubMed
    1. Lu G, Zhao W, Rao D, Zhang S, Zhou M, Xu S (2019) Knockdown of long noncoding RNA WNT5A-AS restores the fate of neural stem cells exposed to sevoflurane via inhibiting WNT5A/Ryk-ROS signaling. Biomed Pharmacother 118:109334. https://doi.org/10.1016/j.biopha.2019.109334 - DOI - PubMed
    1. Nie Y, Li S, Yan T, Ma Y, Ni C, Wang H, Zheng H (2020) Propofol attenuates isoflurane-induced neurotoxicity and cognitive impairment in fetal and offspring mice. Anesth Analg 131(5):1616–1625. https://doi.org/10.1213/ane.0000000000004955 - DOI - PubMed

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