Resident vascular endothelial progenitor definition and function: the age of reckoning

Abstract

The cardiovascular system is composed around the central function of the endothelium that lines the inner surfaces of its vessels. In recent years, the existence of a progenitor population within the endothelium has been validated through the study of endothelial colony-forming cells (ECFCs) in human peripheral blood and certain vascular beds. However, our knowledge on endothelial populations in vivo that can give rise to ECFCs in culture has been limited. In this review we report and analyse recent attempts at describing progenitor populations in vivo from murine studies that reflect the self-renewal and stemness capacity observed in ECFCs. We pinpoint seminal discoveries within the field, which have phenotypically defined, and functionally scrutinised these endothelial progenitors. Furthermore, we review recent publications utilising single-cell sequencing technologies to better understand the endothelium in homeostasis and pathology.

Keywords: Angiogenesis; Endothelium; Heterogeneity; Neovascularisation; Progenitors.

Fig. 1

Phenotypic characterisation and isolation of murine endothelial progenitor cells (EPCs). 5 seminal papers have demonstrated that murine endothelial progenitor cells (EPCs) can be isolated and passaged in culture for an extended period. We summarise the groups which have discovered murine EPCs in various organ beds