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. 2021 Sep 21;93(37):12786-12792.
doi: 10.1021/acs.analchem.1c03075. Epub 2021 Sep 10.

Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH

Liam T Wilson  1 , William J Tipping  2 , Corinna Wetherill  2 , Zoë Henley  3 , Karen Faulds  2 , Duncan Graham  2 , Simon P Mackay  4 , Nicholas C O Tomkinson  1
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Free article

Mitokyne: A Ratiometric Raman Probe for Mitochondrial pH

Liam T Wilson et al. Anal Chem. .
Free article

Abstract

Mitochondrial pH (pHmito) is intimately related to mitochondrial function, and aberrant values for pHmito are linked to several disease states. We report the design, synthesis, and application of mitokyne 1-the first small molecule pHmito sensor for stimulated Raman scattering (SRS) microscopy. This ratiometric probe can determine subtle changes in pHmito in response to external stimuli and the inhibition of both the electron transport chain and ATP synthase with small molecule inhibitors. In addition, 1 was also used to monitor mitochondrial dynamics in a time-resolved manner with subcellular spatial resolution during mitophagy providing a powerful tool for dissecting the molecular and cell biology of this critical organelle.

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