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. 2021 Sep;26(36):2100767.
doi: 10.2807/1560-7917.ES.2021.26.36.2100767.

Novel vancomycin resistance gene cluster in Enterococcus faecium ST1486, Belgium, June 2021

Basil Britto Xavier  1   2 , Jasmine Coppens  3 , Sien De Koster  1   2 , Sahaya Glingston Rajakani  1   2 , Sam Van Goethem  3 , Samy Mzougui  4 , Ahalieyah Anantharajah  4 , Christine Lammens  1   2 , Katherine Loens  3   5 , Youri Glupczynski  1   2 , Herman Goossens  1   2   3   5 , Veerle Matheeussen  3   5
Affiliations

Novel vancomycin resistance gene cluster in Enterococcus faecium ST1486, Belgium, June 2021

Basil Britto Xavier et al. Euro Surveill. 2021 Sep.

Abstract

We identified a novel van gene cluster in a clinical Enterococcus faecium isolate with vancomycin minimum inhibitory concentration (MIC) of 4 µg/mL. The ligase gene, vanP, was part of a van operon cluster of 4,589 bp on a putative novel integrative conjugative element located in a ca 98 kb genomic region presumed to be acquired by horizontal gene transfer from Clostridiumscidens and Roseburia sp. 499. Screening for van genes in E. faecium strains with borderline susceptibility to vancomycin is important.

Keywords: :E. faecium; CC17; ST1486; novel van gene cluster; vanP.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure 1
Figure 1
Genetic organisation of a novel van gene cluster in a putative ICE element, clinical isolate of Enterococcus faecium, Belgium, June 2021
Figure 2
Figure 2
VanP ligase protein sequence compared parental strain with passaged strains, clinical Enterococcus faecium isolate, Belgium, June 2021
Figure 3
Figure 3
Phylogenetic relatedness of D-Ala-D-X ligases from different bacterial species
Figure 4
Figure 4
Minimum spanning tree comparing core genomic allelic profiles in association with VRE clinical strains isolated in the past 5 years, Belgium 2017–2021
See this image and copyright information in PMC

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