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. 2022 Jul;17(7):e2100304.
doi: 10.1002/biot.202100304. Epub 2021 Sep 23.

CRISPR-Cas enzymes: The toolkit revolutionizing diagnostics

Matthew S Verosloff  1 , Sarah J Shapiro  1 , Elizabeth M Hawkins  1 , Emel Alpay  1 , Deepika Verma  1 , Emma G Stanfield  1 , Lior Kreindler  1 , Sonal Jain  1 , Bridget McKay  1 , Shae A Hubbell  1 , Carley G Hendriks  1 , Benjamin A Blizard  1 , James P Broughton  1 , Janice S Chen  1
Affiliations

CRISPR-Cas enzymes: The toolkit revolutionizing diagnostics

Matthew S Verosloff et al. Biotechnol J. 2022 Jul.

Abstract

The programmable nature of sequence-specific targeting by CRISPR-Cas nucleases has revolutionized a wide range of genomic applications and is now emerging as a method for nucleic acid detection. We explore how the diversity of CRISPR systems and their fundamental mechanisms have given rise to a wave of new methods for target recognition and readout. These cross-disciplinary advances found at the intersection of CRISPR biology and engineering have led to the ability to rapidly generate solutions for emerging global challenges like the COVID-19 pandemic. We further discuss the advances and potential for CRISPR-based detection to have an impact across a continuum of diagnostic applications.

Keywords: CRISPR diagnostics; CRISPR-Cas proteins; biochemistry; bioengineering; nucleic acid detection.

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